General Information

    • DRAMP ID

    • DRAMP21006

    • Peptide Name

    • [G1K, K8R]cGm (Derived from Gm)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KCRRLCYRQRCVTYCRGR

    • Sequence Length

    • 18

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antitumor

    • Target Organism

      • [Ref.28741926]Gram-positive bacteria : Staphylococcuss aureus ATCC 25923 (MIC=2 μM);
      • Gram-negative bacteria : Escherichia coli ATCC 25922 (MIC=0.5-1 μM), Escherichia coli CGSC 5167 (MIC<0.015 μM), Klebsiella pneumoniae ATCC 700603 (MIC=8 μM), Acinetobacter baumannii ATCC 19606 (MIC=1-2 μM), Pseudomonas aeruginosa ATCC 27853 (MIC<0.25 μM), Helicobacter pylori ATCC 43504 (MIC>80 μM);
      • Fungi : Candida albicans ATCC 90028 (MIC=2 μM), Cryptococcus neoformans ATCC 208821 (MIC=0.125-0.25 μM)

    • Hemolytic Activity

      • [Ref.28741926] 0% hemolysis at 0.5 μM , 2.5% hemolysis at 0.6 μM , 20% hemolysis at 8 μM , 43.3-56.9% hemolysis at 64 μM , 50% hemolysis at 100 μM against human red blood cells

    • Cytotoxicity

      • [Ref.28741926] CC50 = 19.5 ± 0.5 μM against CRL-1739, CC50 = 1.7 ± 0.1 μM against MM96L, CC50 = 9.0 ± 0.5 μM against HFF-1, CC50 = 44.2 ± 2.2μM against HeLa, CC50 = 6.7 ± 0.7 μM against MCF-7, CC50 = 2.1 ± 0.2 μM against K-562, CC50 = 9.0 ± 1.1μM against HL-60;CC50=5.1 ± 0.3μM against PBMCs.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys2 and Cys15, Cys6 and Cys11.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21006 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21006.

Physicochemical Information

    • Formula

    • C94H162N38O23S4

    • Absent Amino Acids

    • ADEFHIMNPSW

    • Common Amino Acids

    • R

    • Mass

    • 2320.8

    • PI

    • 10.33

    • Basic Residues

    • 7

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 2

    • Net Charge

    • +7

    • Boman Index

    • -8844

    • Hydrophobicity

    • -1.117

    • Aliphatic Index

    • 37.78

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 3230

    • Absorbance 280nm

    • 190

    • Polar Residues

    • 8

DRAMP21006

DRAMP21006 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Cryptococcus neoformans

Literature Information

  • ·Literature 1

    • Title

    • Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties

    • Reference

    • ACS Chem Biol. 2017 Sep 15;12(9):2324-2334. doi: 10.1021/acschembio.7b00459.?

    • Author

    • Sonia Troeira Henriques, Nicole Lawrence, Stephanie Chaousis, Anjaneya S. Ravipati, Olivier Cheneval,Aurelie H. Benfield, Alysha G. Elliott, Angela Maria Kavanagh, Matthew A. Cooper, Lai Yue Chan,Yen-Hua Huang, and David J. Craik