General Information
-
DRAMP ID
- DRAMP21007
-
Peptide Name
- [C/U]cGm (Derived from Gm)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- GURRLUYKQRUVTYURGR
-
Sequence Length
- 18
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antitumor
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Target Organism
-
- [Ref.28741926]Gram-positive bacteria : Staphylococcuss aureus ATCC 25923 (MIC=16 μM);
- Gram-negative bacteria : Escherichia coli ATCC 25922 (MIC=4-8 μM), Escherichia coli CGSC 5167 (MIC=0.03-0.06 μM), Klebsiella pneumoniae ATCC 700603 (MIC=8-16 μM), Acinetobacter baumannii ATCC 19606 (MIC=4 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=2-4 μM), Helicobacter pylori ATCC 43504 (MIC>80 μM);
- Fungi : Candida albicans ATCC 90028 (MIC=4-8 μM), Cryptococcus neoformans ATCC 208821 (MIC=0.5-1 μM)
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Hemolytic Activity
-
- [Ref.28741926] 32.3-33.3% hemolysis at 64 μM against human red blood cells
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Cytotoxicity
-
- [Ref.28741926] CC50 = 51.0 ± 4.6 μM against CRL-1739, CC50 = 4.6 ± 0.2 μM against MM96L, CC50 = 15.6 ± 3.6 μM against HFF-1, CC50 > 64 μM against HeLa, CC50 = 37.5 ± 3.3 μM against MCF-7, CC50 = 1.4 ± 0.2 μM against K-562, CC50 = 38.5 ± 4.8 μM against HL-60;CC50=15.5 ± 0.8μM against PBMCs.
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- No specific N-terminal
-
C-terminal Modification
- No specific C-terminal
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between SeCys2 and SeCys15, SeCys6 and SeCys11. The 'U' in sequence is SelenoCys.
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Stereochemistry
- L
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Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21007.
Physicochemical Information
-
Formula
- C78H125N31O15
Absent Amino Acids
- ACDEFHIMNPSW
Common Amino Acids
- R
Mass
- 2409.27
PI
- 11.84
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 2
Net Charge
- +6
-
Boman Index
- -7770
Hydrophobicity
- -1.444
Aliphatic Index
- 37.78
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 2980
Absorbance 280nm
- 175.29
Polar Residues
- 5
DRAMP21007
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Cryptococcus neoformans
Literature Information
- ·Literature 1
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Title
- Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
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Pubmed ID
- 28741926
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Reference
- ACS Chem Biol. 2017 Sep 15;12(9):2324-2334. doi: 10.1021/acschembio.7b00459.?
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Author
- Sonia Troeira Henriques, Nicole Lawrence, Stephanie Chaousis, Anjaneya S. Ravipati, Olivier Cheneval,Aurelie H. Benfield, Alysha G. Elliott, Angela Maria Kavanagh, Matthew A. Cooper, Lai Yue Chan,Yen-Hua Huang, and David J. Craik