General Information
-
DRAMP ID
- DRAMP21010
-
Peptide Name
- [G1K, L5Y, K8R]cGm (Derived from Gm)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- KCRRYCYRQRCVTYCRGR
-
Sequence Length
- 18
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antitumor
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Target Organism
-
- [Ref.28741926]Gram-positive bacteria : Staphylococcuss aureus ATCC 25923 (MIC=8 μM);
- Gram-negative bacteria : Escherichia coli ATCC 25922 (MIC=0.5-1 μM), Klebsiella pneumoniae ATCC 700603 (MIC=4-8 μM), Acinetobacter baumannii ATCC 19606 (MIC=1-2 μM), Pseudomonas aeruginosa ATCC 27853 (MIC<0.25-0.5 μM);
- Fungi : Candida albicans ATCC 90028 (MIC=4 μM), Cryptococcus neoformans ATCC 208821 (MIC=0.5 μM)
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Hemolytic Activity
-
- [Ref.28741926] 20.6-32% hemolysis at 64 μM against human red blood cells
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Cytotoxicity
-
- [Ref.28741926] CC50 = 26.5 ± 2.4 μM against CRL-1739, CC50 = 2.0 ± 0.1 μM against MM96L, CC50 = 14.7 ± 1.5 μM against HFF-1, CC50 = 20.9 ± 1.4 μM against HeLa, CC50 = 15.2 ± 1.9 μM against MCF-7, CC50 = 1.3 ± 0.1 μM against K-562, CC50 = 15.7 ±1.1μM against HL-60;CC50=10.4 ± 1.0μM against PBMCs.
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- No specific N-terminal
-
C-terminal Modification
- No specific C-terminal
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys2 and Cys15, Cys6 and Cys11.
-
Stereochemistry
- L
-
Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21010.
Physicochemical Information
-
Formula
- C97H160N38O24S4
Absent Amino Acids
- ADEFHILMNPSW
Common Amino Acids
- R
Mass
- 2370.82
PI
- 10.1
Basic Residues
- 7
Acidic Residues
- 0
Hydrophobic Residues
- 1
Net Charge
- +7
-
Boman Index
- -9350
Hydrophobicity
- -1.4
Aliphatic Index
- 16.11
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 4720
Absorbance 280nm
- 277.65
Polar Residues
- 9
DRAMP21010
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Cryptococcus neoformans
Literature Information
- ·Literature 1
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Title
- Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
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Pubmed ID
- 28741926
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Reference
- ACS Chem Biol. 2017 Sep 15;12(9):2324-2334. doi: 10.1021/acschembio.7b00459.?
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Author
- Sonia Troeira Henriques, Nicole Lawrence, Stephanie Chaousis, Anjaneya S. Ravipati, Olivier Cheneval,Aurelie H. Benfield, Alysha G. Elliott, Angela Maria Kavanagh, Matthew A. Cooper, Lai Yue Chan,Yen-Hua Huang, and David J. Craik