General Information
-
DRAMP ID
- DRAMP21011
-
Peptide Name
- [C/U, G1K, L5Y, K8R]cGm (Derived from Gm)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
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Sequence
- KURRYUYRQRUVTYURGR
-
Sequence Length
- 18
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antitumor
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Target Organism
-
- [Ref.28741926]Gram-positive bacteria : Staphylococcuss aureus ATCC 25923 (MIC=8 μM);
- Gram-negative bacteria : Escherichia coli ATCC 25922 (MIC=1-2 μM), Klebsiella pneumoniae ATCC 700603 (MIC=4-16 μM), Acinetobacter baumannii ATCC 19606 (MIC=1-2 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=1 μM);
- Fungi : Candida albicans ATCC 90028 (MIC=4 μM), Cryptococcus neoformans ATCC 208821 (MIC=0.5-1 μM)
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Hemolytic Activity
-
- [Ref.28741926] 17.8-24% hemolysis at 64 μM against human red blood cells
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Cytotoxicity
-
- [Ref.28741926] CC50 = 46.2 ± 7.6 μM against CRL-1739, CC50 = 2.3 ± 0.2 μM against MM96L, CC50 = 30.8 ± 2.0 μM against HFF-1, CC50 = 36.2 ± 2.8 μM against HeLa, CC50 = 29.0 ± 3.7 μM against MCF-7, CC50 = 6.4 ± 0.6 μM against K-562, CC50 = 33.3 ±7.4μM against HL-60;CC50=9.5 ± 0.5μM against PBMCs.
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- No specific N-terminal
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C-terminal Modification
- No specific C-terminal
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between SeCys2 and SeCys15, SeCys6 and SeCys11. The 'U' in sequence is SelenoCys.
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Stereochemistry
- L
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Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21011.
Physicochemical Information
-
Formula
- C85H132N34O16
Absent Amino Acids
- ACDEFHILMNPSW
Common Amino Acids
- R
Mass
- 2558.42
PI
- 11.72
Basic Residues
- 7
Acidic Residues
- 0
Hydrophobic Residues
- 1
Net Charge
- +7
-
Boman Index
- -9862
Hydrophobicity
- -1.956
Aliphatic Index
- 16.11
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 4470
Absorbance 280nm
- 262.94
Polar Residues
- 5
DRAMP21011
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
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Title
- Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
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Pubmed ID
- 28741926
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Reference
- ACS Chem Biol. 2017 Sep 15;12(9):2324-2334. doi: 10.1021/acschembio.7b00459.?
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Author
- Sonia Troeira Henriques, Nicole Lawrence, Stephanie Chaousis, Anjaneya S. Ravipati, Olivier Cheneval,Aurelie H. Benfield, Alysha G. Elliott, Angela Maria Kavanagh, Matthew A. Cooper, Lai Yue Chan,Yen-Hua Huang, and David J. Craik