General Information

    • DRAMP ID

    • DRAMP21012

    • Peptide Name

    • NK-2 (Mammals, Animals)

    • Source

    • Mammal

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KILRGVCKKIMRTFLRRISKDILTGKK

    • Sequence Length

    • 27

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antitumor

    • Target Organism

      • [Ref.29043494]Gram-positive bacteria : Staphylococcus aureus (MIC=2 μM), Staphylococcus epidermidis (MIC=2 μM);
      • Gram-negative bacteria : Escherichia coli (MIC=4 μM), Pseudomonas aeruginosa (MIC=8 μM)

    • Hemolytic Activity

      • [Ref.29043494] 2.5% hemolysis at 10 μg/ml , 8% hemolysis at 25 μg/ml , 22% hemolysis at 50 μg/ml , 32.5% hemolysis at 75 μg/ml , 36% hemolysis at 150 μg/ml , 38% hemolysis at 300 μg/ml against human red blood cells

    • Cytotoxicity

      • [Ref.29043494] IC50 = 25.1 μM against NK-2.
      • IC50 = 8.4 μM against EJ.
      • IC50 = 4.1 μM against PC-3.
      • IC50 = 10.1 μM against T24.
      • IC50 = 13.7 μM against HL-60.
      • The proliferation inhibition activity on K562 is not significant.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21012 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21012.

Physicochemical Information

    • Formula

    • C143H261N45O33S2

    • Absent Amino Acids

    • AEHNPQWY

    • Common Amino Acids

    • K

    • Mass

    • 3203.05

    • PI

    • 11.6

    • Basic Residues

    • 10

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +9

    • Boman Index

    • -6327

    • Hydrophobicity

    • -0.263

    • Aliphatic Index

    • 111.85

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 6

DRAMP21012

DRAMP21012 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Cryptococcus neoformans

Literature Information

  • ·Literature 1

    • Title

    • Influence of Proline Substitution on the Bioactivity of Mammalian-Derived Antimicrobial Peptide NK-2

    • Reference

    • Probiotics Antimicrob Proteins. 2018 Mar;10(1):118-127. doi: 10.1007/s12602-017-9335-1.

    • Author

    • Jiexi Yan & Xiaolei Liang & Chang Liu & Yuemei Cheng & Lanxia Zhou & Kairong Wang & Li Zhao