General Information
-
DRAMP ID
- DRAMP21014
-
Peptide Name
- NK-dpro (Derived from NK-2)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- KILPGVCKKIMRPFLRRISKDILTGKK
-
Sequence Length
- 27
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antitumor
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Target Organism
-
- [Ref.29043494]Gram-positive bacteria : Staphylococcus aureus (MIC=8 μM), Staphylococcus epidermidis (MIC=8 μM);
- Gram-negative bacteria : Escherichia coli (MIC=8 μM), Pseudomonas aeruginosa (MIC=32 μM)
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Hemolytic Activity
-
- [Ref.29043494] 0% hemolysis at 10 μg/ml , 2% hemolysis at 25 μg/ml , 3% hemolysis at 50 μg/ml , 4.5% hemolysis at 75 μg/ml , 10% hemolysis at 150 μg/ml , 20% hemolysis at 300 μg/ml against human red blood cells
-
Cytotoxicity
-
- [Ref.29043494] IC50 = 32.5 μM against NK-2.
- IC50 = 28.7 μM against EJ.
- IC50 = 9.6 μM against PC-3.
- IC50 = 39.6 μM against T24.
- IC50 = 48.4 μM against HL-60.
- The proliferation inhibition activity on K562 is not significant.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21014.
Physicochemical Information
-
Formula
- C143H256N42O32S2
Absent Amino Acids
- AEHNQWY
Common Amino Acids
- K
Mass
- 3139.99
PI
- 11.17
Basic Residues
- 9
Acidic Residues
- 1
Hydrophobic Residues
- 9
Net Charge
- +8
-
Boman Index
- -4578
Hydrophobicity
- -0.189
Aliphatic Index
- 111.85
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 5
DRAMP21014
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- Influence of Proline Substitution on the Bioactivity of Mammalian-Derived Antimicrobial Peptide NK-2
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Pubmed ID
- 29043494
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Reference
- Probiotics Antimicrob Proteins. 2018 Mar;10(1):118-127. doi: 10.1007/s12602-017-9335-1.
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Author
- Jiexi Yan & Xiaolei Liang & Chang Liu & Yuemei Cheng & Lanxia Zhou & Kairong Wang & Li Zhao