General Information
-
DRAMP ID
- DRAMP21022
-
Peptide Name
- A (A1R, A8R, I17K) (Derived from AR-23)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- RIGSILGRLAKGLPTLKSWIKNR
-
Sequence Length
- 23
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.27271216]Gram-positive bacteria : Staphylococcuss aureus (MIC=100 μM), Staphylococcus epidermidis (MIC=6.25 μM), Bacillus subtilis (MIC=12.5 μM);
- Gram-negative bacteria : Escherichia coli (MIC=12.5 μM), Pseudomonas aeruginosa (MIC=12.5 μM), Klebsiella pneumoniae (MIC=12.5 μM)
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Hemolytic Activity
-
- [Ref.27271216] 0% hemolysis at 40 μM , 1% hemolysis at 60 μM , 2% hemolysis at 80 μM , 2% hemolysis at 100 μM against human red blood cells
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Cytotoxicity
-
- [Ref.27271216] In L929 cells, the cell viability is 96%, 95%, 92%, 68% and 31.4% at peptide concentrations of 3.12, 6.25, 12.5, 25 and 50 μM
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
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C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21022.
Physicochemical Information
-
Formula
- C117H205N37O28
Absent Amino Acids
- CDEFHMQVY
Common Amino Acids
- L
Mass
- 2578.15
PI
- 12.31
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 9
Net Charge
- +6
-
Boman Index
- -3602
Hydrophobicity
- -0.183
Aliphatic Index
- 123.04
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 250
Polar Residues
- 7
DRAMP21022
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- Design of an α-helical antimicrobial peptide with improved cell selective and potent anti-biofilm activity
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Pubmed ID
- 27271216
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Reference
- Sci Rep. 2016 Jun 8;6:27394. doi: 10.1038/srep27394.
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Author
- Shi-KunZhang, Jin-wen Song, FengGong, Su-Bo Li, Hong-Yu Chang, Hui-MinXie, Hong-WeiGao, Ying-XiaTan & Shou-Ping Ji