General Information

    • DRAMP ID

    • DRAMP21025

    • Peptide Name

    • StigA25 (Derived from Stigmurin)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • FFSLIPSLVKKLIKAFK

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.30709056]Gram-positive bacteria : Staphylococcus aureus (ATCC 29213)(MIC=1.2 μM), Staphylococcus epidermidis (ATCC 12228)(MIC=2.3 μM), Enterococcus faecalis (ATCC 4028)(MIC=4.7 μM);
      • Gram-negative bacteria : Pseudomonas aeruginosa (ATCC 27853)(MIC=4.7 μM), Escherichia coli (ATCC 25922)(MIC=2.3 μM), Enterobacter cloacae (ATCC 13047)(MIC=18.8 μM);
      • Fungi : Candida albicans (ATCC 90028)(MIC=9.4 μM), Candida glabrata (ATCC 90030)(MIC=9.4 μM), Candida krusei (ATCC 6258)(MIC=9.4 μM)

    • Hemolytic Activity

      • [Ref.30709056] 1% hemolysis at 1.2 μg/ml , 5% hemolysis at 2.3 μg/ml , 9% hemolysis at 4.7 μg/ml , 19% hemolysis at 9.4 μg/ml , 33% hemolysis at 18.8 μg/ml , 60% hemolysis at 37.5 μg/ml , 78% hemolysis at 75 μg/ml , 93% hemolysis at 150 μg/ml against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21025 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21025.

Physicochemical Information

    • Formula

    • C100H163N21O20

    • Absent Amino Acids

    • CDEGHMNQRTWY

    • Common Amino Acids

    • K

    • Mass

    • 1979.52

    • PI

    • 10.48

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +4

    • Boman Index

    • 1039

    • Hydrophobicity

    • 0.941

    • Aliphatic Index

    • 137.65

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP21025

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Candida glabrata, Candida krusei

Literature Information

  • ·Literature 1

    • Title

    • Potent and Broad-Spectrum Antimicrobial Activity of Analogs from the Scorpion Peptide Stigmurin

    • Reference

    • Int J Mol Sci. 2019 Jan 31;20(3). pii: E623. doi: 10.3390/ijms20030623.

    • Author

    • Bruno Amorim-Carmo , Alessandra Daniele-Silva , Adriana M. S. Parente ,Allanny A. Furtado , Eneas Carvalho , Johny W. F. Oliveira , Elizabeth C. G. Santos , Marcelo S. Silva , Sérgio R. B. Silva , Arnóbio A. Silva-Júnior , Norberto K. Monteiro and Matheus F. Fernandes-Pedrosa