General Information

    • DRAMP ID

    • DRAMP21027

    • Peptide Name

    • K5, 17-DPS3 (Derived from dermaseptin-PS3 (DPS3))

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ALWKKILKNAGKAALNKINQIVQ

    • Sequence Length

    • 23

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.30087268]Gram-positive bacteria : Staphylococcuss aureus (NCTC 10788)(MIC=8 μM);
      • Gram-negative bacteria : Escherichia coli (NCTC 10418)(MIC=8 μM);
      • Fungi : Candida albicans (NCYC 1467)(MIC=4 μM)

    • Hemolytic Activity

      • [Ref.30087268] 8% hemolysis at 0 μM , 40% hemolysis at 1 μM , 90% hemolysis at 2 μM against horse red blood cells

    • Cytotoxicity

      • [Ref.30087268] IC50 = 18.20 μM for H157.
      • IC50 = 18.20 μM for PC3.
      • IC50 = 132.10 μM for HMEC-1

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix

    • Structure Description

    • These three peptides existed in random coils in aqueous solution, they all adopted

    • Helical Wheel Diagram

    • DRAMP21027 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21027.

Physicochemical Information

    • Formula

    • C118H204N34O29

    • Absent Amino Acids

    • CDEFHMPRSTY

    • Common Amino Acids

    • K

    • Mass

    • 2563.13

    • PI

    • 10.6

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +5

    • Boman Index

    • -1468

    • Hydrophobicity

    • -0.087

    • Aliphatic Index

    • 131.74

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 250

    • Polar Residues

    • 4

DRAMP21027

DRAMP21027 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans

Literature Information

  • ·Literature 1

    • Title

    • Biological Activities of Cationicity-Enhanced and Hydrophobicity-Optimized Analogues of an Antimicrobial Peptide, Dermaseptin-PS3, from the Skin Secretion of Phyllomedusa sauvagii

    • Reference

    • Toxins (Basel). 2018 Aug 7;10(8). pii: E320. doi: 10.3390/toxins10080320.

    • Author

    • Yining Tan ,Xiaoling Chen , Chengbang Ma , Xinping Xi ,Lei Wang , Mei Zhou ,James F. Burrows ,Hang Fai Kwok 2 ID and Tianbao Chen