General Information

    • DRAMP ID

    • DRAMP21037

    • Peptide Name

    • PapMA-k (Derived from Papiliocin and Magainin 2)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RWKIFKKIkKFLHSAKKF

    • Sequence Length

    • 18

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.26053120] Gram-positive bacteria : Bacillus subtilis(MIC=4.0 μM), Enterococcus faecalis(MIC=16 μM), Staphylococcus aureus(MIC=4.0 μM), ;
      • Gram-negative bacteria : Escherichia coli(MIC=2.0 μM), Pseudomonas aeruginosa(MIC=4.0 μM), Salmonella typhimurium(MIC=2.0 μM)

    • Hemolytic Activity

      • [Ref.26053120] MHC=200 μM against human red blood cells

    • Cytotoxicity

      • [Ref.26053120] IC50 = 42 μM against mouse macrophage-derived RAW264.7 cells.
      • IC50 = 39 μM for NIH3T3 fibroblasts.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • The 'k' in sequence is lysine peptoid

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • PapMA and PapMA-k exhibited α-helical structures in 50 mM DPC micelles and 50% TFE/H3O but displayed unordered structures in aqueous solution.

    • Helical Wheel Diagram

    • DRAMP21037 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21037.

Physicochemical Information

    • Formula

    • C110H171N29O18

    • Absent Amino Acids

    • CDEGMNPQTVY

    • Common Amino Acids

    • K

    • Mass

    • 2333.94

    • PI

    • 11.47

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +8

    • Boman Index

    • -2844

    • Hydrophobicity

    • -0.544

    • Aliphatic Index

    • 70.56

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 323.53

    • Polar Residues

    • 1

DRAMP21037

DRAMP21037 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Peptoid-Substituted Hybrid Antimicrobial Peptide Derived from Papiliocin and Magainin 2 with Enhanced Bacterial Selectivity and Anti-inflammatory Activity

    • Reference

    • Biochemistry. 2015 Jun 30;54(25):3921-31. doi: 10.1021/acs.biochem.5b00392.

    • Author

    • Areum Shin, Eunjung Lee, Dasom Jeon, Young-Guen Park, Jeong Kyu Bang, Yong-Sun Park, Song Yub Shin, and Yangmee Kim