General Information
-
DRAMP ID
- DRAMP21038
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Peptide Name
- analog 1 (Derived from Ib-AMP1)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- WGRRGWGPGRRYVRN
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Sequence Length
- 15
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.19778562] Gram-positive bacteria : Bacillus subtilis(KCTC 3068)(MIC=8 μM), Staphylococcus epidermidis(KCTC 1917)(MIC=8 μM), Staphylococcus aureus(KCTC 1621)(MIC=4 μM), Staphylococcus aureus (MRSA)(CCARM 3543)(MIC=2 μM);
- Gram-negative bacteria : Escherichia coli(KCTC 1682)(MIC=16 μM), Pseudomonas aeruginosa(KCTC 1637)(MIC=16 μM), Salmonella typhimurium(KCTC 1926)(MIC=4 μM), Pseudomonas aeruginosa (MDRPA)(CCARM 2095)(MIC=16 μM)
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Hemolytic Activity
-
- [Ref.19778562] MHC>400 μM against human red blood cells
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
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Structure
- Random coil, beta turn
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Structure Description
- In sodium phosphate buffer, the CD spectra of Ib-AMP1 and all linear analogs showed a large absorbance in the region of 190-200 nm, indicating a random coil structure. The spectra of Ib-AMP1 and analogs 1, 2, 3, and 4 became significantly more b-turn in 30 mM SDS micelles
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21038.
Physicochemical Information
-
Formula
- C83H125N33O18
Absent Amino Acids
- ACDEFHIKLMQST
Common Amino Acids
- R
Mass
- 1873.12
PI
- 12.18
Basic Residues
- 5
Acidic Residues
- 0
Hydrophobic Residues
- 3
Net Charge
- +5
-
Boman Index
- -6892
Hydrophobicity
- -1.873
Aliphatic Index
- 19.33
Half Life
-
- Mammalian:2.8 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 12490
Absorbance 280nm
- 892.14
Polar Residues
- 6
DRAMP21038
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
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Title
- Antimicrobial specificity and mechanism of action of disulfide-removed linear analogs of the plant-derived Cys-rich antimicrobial peptide Ib-AMP1
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Pubmed ID
- 19778562
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Reference
- Peptides. 2009 Dec;30(12):2144-9. doi: 10.1016/j.peptides.2009.09.020.
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Author
- Peng Wang , Jeong-Kyu Bang , Hak Jun Kim , Jin-Kyoung Kim , Yangmee Kim , Song Yub Shin