General Information
-
DRAMP ID
- DRAMP21070
-
Peptide Name
- TP1[V6R, R9A] (Derived from TP1)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- KWCFRRCYAGICYRRCR
-
Sequence Length
- 17
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
-
Target Organism
-
- [Ref.28960954] Gram-positive bacteria : Bacillus subtilis ATCC 6051(MIC=0.25 μg/ml), Staphylococcus aureus ATCC 43300 (MRSA)(MIC>16 μg/ml);
- Gram-negative bacteria : Escherichia coli ATCC 25922(MIC=0.5 μg/ml), Klebsiella pneumoniae ATCC 13883(MIC>16 μg/ml), Klebsiella pneumoniae ATCC 700603 (MDR)(MIC=16 μg/ml), Klebsiella pneumoniae BAA 2146 (NDM-1 pos)(MIC>16 μg/ml), Klebsiella pneumoniae clinical isolate 100650661 : 1 (XDR;PmxR)(MIC>16 μg/ml), Acinetobacter baumannii ATCC 19606(MIC=16 μg/ml), Acinetobacter baumannii clinical isolate 100734512 : 2 (XDR;PmxR)(MIC>16 μg/ml), Pseudomonas aeruginosa ATCC 27853(MIC>16 μg/ml), Pseudomonas aeruginosa FADDIFA070 (PmxR)(MIC>16 μg/ml);
- Fungi : Candida albicans ATCC 90028(MIC>16 μg/ml), Cryptococcus neoformans ATCC 208821(MIC=4 μg/ml)
-
Hemolytic Activity
-
- [Ref.28960954] MHC10=11 μg/ml against human red blood cells
-
Cytotoxicity
-
- [Ref.28960954] The peptide did not show cytotoxicity against HEK293 cells at the highest tested concentration, 100 μg/ml.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys3 and Cys16, Cys7 and Cys12
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21070.
Physicochemical Information
-
Formula
- C97H148N34O19S4
Absent Amino Acids
- DEHLMNPQSTV
Common Amino Acids
- R
Mass
- 2240.71
PI
- 9.93
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 4
Net Charge
- +6
-
Boman Index
- -6233
Hydrophobicity
- -0.622
Aliphatic Index
- 27.22
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 8730
Absorbance 280nm
- 513.53
Polar Residues
- 7
DRAMP21070
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Cryptococcus neoformans
Literature Information
- ·Literature 1
-
Title
- Structure-Activity and -Toxicity Relationships of the Antimicrobial Peptide Tachyplesin-21
-
Pubmed ID
- 28960954
-
Reference
- ACS Infect Dis. 2017 Dec 8;3(12):917-926. doi: 10.1021/acsinfecdis.7b00123.
-
Author
- Ingrid A. Edwards , Alysha G. Elliott , Angela M. Kavanagh , Mark A. T. Blaskovich , and Matthew A. Cooper