General Information

    • DRAMP ID

    • DRAMP21076

    • Peptide Name

    • TP1[F4A, Y8A, I11A] (Derived from TP1)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KWCARVCARGACYRRCR

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.28960954] Gram-positive bacteria : Bacillus subtilis ATCC 6051(MIC>16 μg/ml), Staphylococcus aureus ATCC 43300 (MRSA)(MIC>16 μg/ml);
      • Gram-negative bacteria : Escherichia coli ATCC 25922(MIC=8 μg/ml), Klebsiella pneumoniae ATCC 13883(MIC>16 μg/ml), Klebsiella pneumoniae ATCC 700603 (MDR)(MIC>16 μg/ml), Klebsiella pneumoniae BAA 2146 (NDM-1 pos)(MIC>16 μg/ml), Klebsiella pneumoniae clinical isolate 100650661 : 1 (XDR;PmxR)(MIC>16 μg/ml), Acinetobacter baumannii ATCC 19606(MIC>16 μg/ml), Acinetobacter baumannii clinical isolate 100734512 : 2 (XDR;PmxR)(MIC>16 μg/ml), Pseudomonas aeruginosa ATCC 27853(MIC>16 μg/ml), Pseudomonas aeruginosa FADDIFA070 (PmxR)(MIC>16 μg/ml);
      • Fungi : Candida albicans ATCC 90028(MIC>16 μg/ml), Cryptococcus neoformans ATCC 208821(MIC>16 μg/ml)

    • Hemolytic Activity

      • [Ref.28960954] MHC10=0.9 μg/ml against human red blood cells

    • Cytotoxicity

      • [Ref.28960954] The peptide did not show cytotoxicity against HEK293 cells at the highest tested concentration, 100 μg/ml.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys3 and Cys16, Cys7 and Cys12

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21076 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21076.

Physicochemical Information

    • Formula

    • C84H138N34O18S4

    • Absent Amino Acids

    • DEFHILMNPQST

    • Common Amino Acids

    • R

    • Mass

    • 2058.49

    • PI

    • 10.16

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +6

    • Boman Index

    • -6243

    • Hydrophobicity

    • -0.522

    • Aliphatic Index

    • 32.78

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 7240

    • Absorbance 280nm

    • 425.88

    • Polar Residues

    • 6

DRAMP21076

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Cryptococcus neoformans

Literature Information

  • ·Literature 1

    • Title

    • Structure-Activity and -Toxicity Relationships of the Antimicrobial Peptide Tachyplesin-27

    • Reference

    • ACS Infect Dis.2017 Dec 8;3(12):917-926. doi: 10.1021/acsinfecdis.7b00123.

    • Author

    • Ingrid A. Edwards , Alysha G. Elliott , Angela M. Kavanagh , Mark A. T. Blaskovich , and Matthew A. Cooper