General Information

    • DRAMP ID

    • DRAMP21079

    • Peptide Name

    • TP1[K1A, Y8A] (Derived from TP1)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • AWCFRVCARGICYRRCR

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.28960954] Gram-positive bacteria : Bacillus subtilis ATCC 6051(MIC=0.5 μg/ml), Staphylococcus aureus ATCC 43300 (MRSA)(MIC=8 μg/ml);
      • Gram-negative bacteria : Escherichia coli ATCC 25922(MIC=0.125 μg/ml), Klebsiella pneumoniae ATCC 13883(MIC=0.25 μg/ml), Klebsiella pneumoniae ATCC 700603 (MDR)(MIC=0.5 μg/ml), Klebsiella pneumoniae BAA 2146 (NDM-1 pos)(MIC=1 μg/ml), Klebsiella pneumoniae clinical isolate 100650661 : 1 (XDR;PmxR)(MIC=8 μg/ml), Acinetobacter baumannii ATCC 19606(MIC=0.25 μg/ml), Acinetobacter baumannii clinical isolate 100734512 : 2 (XDR;PmxR)(MIC=0.5 μg/ml), Pseudomonas aeruginosa ATCC 27853(MIC=1 μg/ml), Pseudomonas aeruginosa FADDIFA070 (PmxR)(MIC=4 μg/ml);
      • Fungi : Candida albicans ATCC 90028(MIC=>16 μg/ml), Cryptococcus neoformans ATCC 208821(MIC=2 μg/ml)

    • Hemolytic Activity

      • [Ref.28960954] MHC10=0.4 μg/ml against human red blood cells

    • Cytotoxicity

      • [Ref.28960954] The peptide did not show cytotoxicity against HEK293 cells at the highest tested concentration, 100 μg/ml.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys3 and Cys16, Cys7 and Cys12

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21079 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21079.

Physicochemical Information

    • Formula

    • C90H141N33O18S4

    • Absent Amino Acids

    • DEHKLMNPQST

    • Common Amino Acids

    • R

    • Mass

    • 2119.58

    • PI

    • 9.88

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +5

    • Boman Index

    • -5079

    • Hydrophobicity

    • 0

    • Aliphatic Index

    • 48.89

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 7240

    • Absorbance 280nm

    • 425.88

    • Polar Residues

    • 6

DRAMP21079

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Cryptococcus neoformans

Literature Information

  • ·Literature 1

    • Title

    • Structure-Activity and -Toxicity Relationships of the Antimicrobial Peptide Tachyplesin-30

    • Reference

    • ACS Infect Dis. 2017 Dec 8;3(12):917-926. doi: 10.1021/acsinfecdis.7b00123.

    • Author

    • Ingrid A. Edwards , Alysha G. Elliott , Angela M. Kavanagh , Mark A. T. Blaskovich , and Matthew A. Cooper