• • DRAMP ID

  • DRAMP21086

• • Peptide Name

  • PR-FO (Derived from PRW4)

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • RFRRLRWKTRWRLKKIRFGRFLRKIRRFRPK

• • Sequence Length

  • 31

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.25818457] Gram-positive bacteria : Staphylococcus aureus 25923(MIC=8.6 mg/L), Staphylococcus aureus 29213(MIC=4.3 mg/L), Staphylococcus epidermidis 12228(MIC=2.2 mg/L), Enterococcus faecalis 29212(MIC=1.1 mg/L), Bacillus subtilis 11060(MIC=2.2 mg/L);
    • Gram-negative bacteria : Escherichia coli 078(MIC=2.2 mg/L), Escherichia coli K88(MIC=4.3 mg/L), Escherichia coli ER2566(MIC=2.2 mg/L), Escherichia coli 44102(MIC=8.6 mg/L), Escherichia coli 1515(MIC=2.2 mg/L), Escherichia coli 25922(MIC=2.2 mg/L), Escherichia coli UB1005(MIC=4.3 mg/L), Salmonella typhimurium 7731(MIC=0.5 mg/L), Salmonella typhimurium 1402(MIC=8.6 mg/L), Pseudomonas aeruginosa 21625(MIC=8.6 mg/L), Pseudomonas aeruginosa 21630(MIC=0.5 mg/L), Pseudomonas aeruginosa 10419(MIC=4.3 mg/L), Pseudomonas aeruginosa 27853(MIC=8.6 mg/L), Salmonella pullorum 7913(MIC=8.6 mg/L)

• • Hemolytic Activity

  • • [Ref.25818457] 0% hemolysis at 9.2 mg/L , 1% hemolysis at 18.4 mg/L , 4% hemolysis at 36.8mg/L , 5% hemolysis at 73.6 mg/L , 10% hemolysis at 147.1 mg/L , 13% hemolysis at 294.3 mg/L against human red blood cells

• • Cytotoxicity

  • • [Ref.25818457] The cell viability of PR-FO is 93%, 89%, 84%, 79%, 74%, 69%, 67%, 64% and 62% at peptide concentrations of 1.1, 2.3, 4.6, 9.2, 18.4, 36.8, 73.6, 147.1 and 294.3 μg/ml.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Random coil, alpha helix, beta Sheet

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP21086.

DRAMP21086

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

• ·Literature 1

• • Title

  • Characterization of cell selectivity, physiological stability and endotoxin neutralization capabilities of a-helix-based peptide amphiphiles

• • Pubmed ID

  • 25818457

• • Reference

  • Biomaterials. 2015 Jun;52:517-30. doi: 10.1016/j.biomaterials.2015.02.063.

• • Author

  • Zhi Ma, Dandan Wei, Ping Yan, Xin Zhu, Anshan Shan , Zhongpeng Bi