General Information

    • DRAMP ID

    • DRAMP21103

    • Peptide Name

    • L-RW (De novo synthesis)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RWTWRGSGRWTWR

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.25683050] Gram-positive bacteria : Staphylococcus aureus(MIC=8 μg/ml), Micrococcus luteus(MIC=16 μg/ml), Bacillus subtilis(MIC=16 μg/ml);
      • Gram-negative bacteria : Escherichia coli(MIC=16 μg/ml), Pseudomonas aeruginosa(MIC=125 μg/ml), Acinetobacter baumannii(MIC=32 μg/ml), Stenotrophomonas maltophilia(MIC>500 μg/ml)

    • Hemolytic Activity

      • [Ref.25683050] 0% hemolysis at 8 mg/L , 3.25% hemolysis at 16 mg/L , 0% hemolysis at 32mg/L , 0% hemolysis at 64 mg/L , 0% hemolysis at 125 mg/L , 0% hemolysis at mg/L , 2.5% hemolysis at 500 mg/L against rabbit red blood cells

    • Cytotoxicity

      • [Ref.25683050] The inhibition ratio of I-RW against human bronchial epithelial cell line BEAS-2B cells is 11.3%, 2.1%, 0.5%, 6.2%, 1.5%, 8.2%, 37.4% and 51.8% at peptide concentrations of 2.5, 5, 10, 20, 40, 80, 160 and 320 μg/ml

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Acylation (Conjugated with acetyl group)

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21103 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21103.

Physicochemical Information

    • Formula

    • C83H115N29O17

    • Absent Amino Acids

    • ACDEFHIKLMNPQVY

    • Common Amino Acids

    • RW

    • Mass

    • 1791.01

    • PI

    • 12.48

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +4

    • Boman Index

    • -5702

    • Hydrophobicity

    • -1.892

    • Aliphatic Index

    • 0

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 22000

    • Absorbance 280nm

    • 1833.33

    • Polar Residues

    • 5

DRAMP21103

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Activity of a novel-designed antimicrobial peptide and its interaction with lipids

    • Reference

    • J Pept Sci. 2015 Apr;21(4):274-82. doi: 10.1002/psc.2728. 

    • Author

    • Lanlan Yu, Qiannan Fan, Xiu Yue, Yexuan Maoa and Lingbo Qu