General Information
-
DRAMP ID
- DRAMP21110
-
Peptide Name
- SLZP (De novo synthesis)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- LRRLLRWLRRLLRR
-
Sequence Length
- 14
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.25069749] Gram-positive bacteria : Staphylococcus aureus (ATCC 25923)(MIC=1.56 μg/ml), Bacillus cereus(MIC=3.15 μg/ml);
- Gram-negative bacteria : E. coli (ATCC 9637)(MIC=25 μg/ml), Pseudomonas aeruginosa (ATCC BAA-427)(MIC=25 μg/ml), Klebsiella pneumoniae (ATCC 27736)(MIC=3.15 μg/ml);
- Fungi : Sporothrix schenckii(MIC=3.15 μg/ml), Trichophyton mentagrophytes(MIC=6.25 μg/ml), Aspergillus fumigatus(MIC=6.25 μg/ml), Candida parapsilosis (ATCC 22019)(MIC=6.25 μg/ml)
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Hemolytic Activity
-
- [Ref.25069749] 15% hemolysis at 10 μg/ml , 25% hemolysis at 20 μg/ml , 30% hemolysis at 30 μg/ml , 40% hemolysis at 40 μg/ml , 50% hemolysis at 50 μg/ml , 55% hemolysis at 60 μg/ml , 70% hemolysis at 70 μg/ml , 80% hemolysis at 80μg/ml against human red blood cells
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Cytotoxicity
-
- [Ref.25069749] The cell viability of murine fibroblast cells (3T3) is 85.9%, 77.5%, 73.7%, 50.0%, 23.7% and 0% at peptide concentrations of 1.5, 3, 4.5, 6 and 9 μM.
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21110.
Physicochemical Information
-
Formula
- C89H162N36O15
Absent Amino Acids
- ACDEFGHIKMNPQSTVY
Common Amino Acids
- R
Mass
- 1976.5
PI
- 12.78
Basic Residues
- 7
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +7
-
Boman Index
- -7259
Hydrophobicity
- -0.686
Aliphatic Index
- 167.14
Half Life
-
- Mammalian:5.5 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 423.08
Polar Residues
- 0
DRAMP21110
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Sporothrix schenckii, Trichophyton mentagrophytes, Aspergillus fumigatus, Candida parapsilosis
Literature Information
- ·Literature 1
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Title
- Design and characterization of short antimicrobial peptides using leucine zipper templates with selectivity towards microorganisms
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Pubmed ID
- 25069749
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Reference
- Amino Acids. 2014 Nov;46(11):2531-43. doi: 10.1007/s00726-014-1802-3.
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Author
- Ahmad A, Azmi S, Srivastava S, Kumar A, Tripathi JK, Mishra NN, Shukla PK, Ghosh JK