General Information

    • DRAMP ID

    • DRAMP21122

    • Peptide Name

    • KR-12-a5 (6-(D)L) (Derived from LL-37)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KRIVKlILKWLR

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.28525841] Gram-positive bacteria : Bacillus subtilis(KCTC 3068)(MIC=4 μM), Staphylococcus epidermidis(KCTC 1917)(MIC=4 μM), Staphylococcus aureus(KCTC1621)(MIC=2 μM);
      • Gram-negative bacteria : Escherichia coli(KCTC 1682)(MIC=4 μM), Pseudomonas aeruginosa(KCTC 1637)(MIC=4 μM), Salmonella typhimurium(KCTC 1926)(MIC=2 μM)

    • Hemolytic Activity

      • [Ref.28525841] MHC10=202 μM against human red blood cells

    • Cytotoxicity

      • [Ref.28525841] The cell viability of mouse macrophage RAW264.7 cells is 100%, 101.9%, 118.9%, 107.5%, 111.3%, 110.6% and 110.6% at peptide concentrations of 0, 4, 8, 16, 32, 64, 128 and 256 μM

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • Mixed(6 is D)

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21122 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21122.

Physicochemical Information

    • Formula

    • C70H123N21O11

    • Absent Amino Acids

    • ACDEFGHMNPQSTY

    • Common Amino Acids

    • K

    • Mass

    • 1566.06

    • PI

    • 12.02

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +5

    • Boman Index

    • -2044

    • Hydrophobicity

    • -0.067

    • Aliphatic Index

    • 154.17

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 500

    • Polar Residues

    • 0

DRAMP21122

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • LL-37-derived short antimicrobial peptide KR-12-a5 and its d-amino acid substituted analogs with cell selectivity, anti-biofilm activity, synergistic effect with conventional antibiotics, and anti-inflammatory activity.

    • Reference

    • Eur J Med Chem. 2017 Aug 18;136:428-441. doi: 10.1016/j.ejmech.2017.05.028.

    • Author

    • Kim EY, Rajasekaran G, Shin SY