General Information

    • DRAMP ID

    • DRAMP21180

    • Peptide Name

    • WG18 (Derived from leucocin A)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • WGEAFSAGVHRLANGGNG

    • Sequence Length

    • 18

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.28002968] Gram-positive bacteria : Staphylococcus aureus 29213(MIC>71.15 μM), Staphylococcus aureus 1.160(MIC>71.15 μM), Listeria monocytogenes 21662(MIC>71.15 μM), Listeria monocytogenes 21633(MIC>71.15 μM), Bacillus cereus 63301(MIC>71.15 μM), Micrococcus luteus 28001(MIC>71.15 μM), Bacillus pumilus 63202(MIC>71.15 μM), Pseudomonas fluorescens 21620(MIC>71.15 μM), MRSA 43300(MIC>71.15 μM);
      • Gram-negative bacteria : Escherichia coli O157 : H7 21530(MIC>71.15 μM), Escherichia coli 25922(MIC>71.15 μM), Escherichia coli 35150(MIC>71.15 μM), Salmonella paratyphi-A 20501(MIC>71.15 μM), Salmonella paratyphi paratyphi-B 21495(MIC>71.15 μM), Salmonella paratyphi paratyphi-C 21512(MIC>71.15 μM), Shigella flexneri 51571(MIC>71.15 μM), Salmonella choleraesuis 13312(MIC>71.15 μM), Salmonella typhimurium 51005(MIC>71.15 μM), Salmonella enteritidis 21527(MIC>71.15 μM), Vibrio parahemolyticus 21617(MIC>71.15 μM);
      • Fungi : Candida albicans 10231(MIC>71.15 μM)

    • Hemolytic Activity

      • [Ref.28002968] MHC10>71.15 μM against human red blood cells

    • Cytotoxicity

      • [Ref.28002968] The cell viability of RAW264.7 cells induced by WG18 is 98.87%, 94.93%, 93.52%, 90.14%, 85.35%, 83.10%, 80.56%, 75.77% and 73.62% at peptide concentrations of 0.25, 0.5, 1, 2, 4, 8, 16, 32 and 64 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix, beta sheet, turn

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21180 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21180.

Physicochemical Information

    • Formula

    • C78H114N26O24

    • Absent Amino Acids

    • CDIKMPQTY

    • Common Amino Acids

    • G

    • Mass

    • 1799.92

    • PI

    • 6.75

    • Basic Residues

    • 2

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +1

    • Boman Index

    • -1867

    • Hydrophobicity

    • -0.317

    • Aliphatic Index

    • 54.44

    • Half Life

      • Mammalian:2.8 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 323.53

    • Polar Residues

    • 8

DRAMP21180

DRAMP21180 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans

Literature Information

  • ·Literature 1

    • Title

    • Insights into the Antimicrobial Activity and Cytotoxicity of Engineered α-Helical PeptideAmphiphiles.

    • Reference

    • J Med Chem. 2016 Dec 22;59(24):10946-10962. doi: 10.1021/acs.jmedchem.6b00922.

    • Author

    • Ma Z, Yang J, Han J, Gao L, Liu H, Lu Z, Zhao H, Bie X