General Information

    • DRAMP ID

    • DRAMP21185

    • Peptide Name

    • WRL2 (Derived from leucocin A)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • WGRAFRRLVRRLARGLRR

    • Sequence Length

    • 18

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.28002968] Gram-positive bacteria : Staphylococcus aureus 29213(MIC=3.49 μM), Staphylococcus aureus 1.160(MIC=1.74 μM), Listeria monocytogenes 21662(MIC=1.74 μM), Listeria monocytogenes 21633(MIC=1.74 μM), Bacillus cereus 63301(MIC=55.78 μM), Micrococcus luteus 28001(MIC=27.89 μM), Bacillus pumilus 63202(MIC=55.78 μM), Pseudomonas fluorescens 21620(MIC=55.78 μM), MRSA 43300(MIC=6.97 μM);
      • Gram-negative bacteria : Escherichia coli O157 : H7 21530(MIC=6.97 μM), Escherichia coli 25922(MIC=27.89 μM), Escherichia coli 35150(MIC=13.94 μM), Salmonella paratyphi-A 20501(MIC=3.49 μM), Salmonella paratyphi paratyphi-B 21495(MIC=6.97 μM), Salmonella paratyphi paratyphi-C 21512(MIC=3.49 μM), Shigella flexneri 51571(MIC=3.49 μM), Salmonella choleraesuis 13312(MIC=13.94 μM), Salmonella typhimurium 51005(MIC=13.94 μM), Salmonella enteritidis 21527(MIC>55.78 μM), Vibrio parahemolyticus 21617(MIC=13.94 μM);
      • Fungi : Candida albicans 10231(MIC=13.94 μM)

    • Hemolytic Activity

      • [Ref.28002968] MHC10>55.78 μM against human red blood cells

    • Cytotoxicity

      • [Ref.28002968] The cell viability of RAW264.7 cells induced by WRL2 is 87.32%, 85.35%, 81.13%, 79.15%, 78.87%, 78.03%, 76.34%, 69.86% and 64.79% at peptide concentrations of 0.25, 0.5, 1, 2, 4, 8, 16, 32 and 64 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix, beta sheet, turn

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21185 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21185.

Physicochemical Information

    • Formula

    • C101H175N43O19

    • Absent Amino Acids

    • CDEHIKMNPQSTY

    • Common Amino Acids

    • R

    • Mass

    • 2295.78

    • PI

    • 12.85

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +8

    • Boman Index

    • -8975

    • Hydrophobicity

    • -0.872

    • Aliphatic Index

    • 92.22

    • Half Life

      • Mammalian:2.8 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 323.53

    • Polar Residues

    • 2

DRAMP21185

DRAMP21185 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans

Literature Information

  • ·Literature 1

    • Title

    • Insights into the Antimicrobial Activity and Cytotoxicity of Engineered α-Helical PeptideAmphiphiles.

    • Reference

    • J Med Chem. 2016 Dec 22;59(24):10946-10962. doi: 10.1021/acs.jmedchem.6b00922.

    • Author

    • Ma Z, Yang J, Han J, Gao L, Liu H, Lu Z, Zhao H, Bie X