General Information
-
DRAMP ID
- DRAMP21235
-
Peptide Name
- FK13-a2 (Derived from FK13)
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Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- WKRIVRWIKRWLR
-
Sequence Length
- 13
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.28161291] Gram-positive bacteria : Bacillus subtilis(KCTC 3068)(MIC=4 μM), Staphylococcus epidermidis(KCTC 1917)(MIC=8 μM), Staphylococcus aureus(KCTC1621)(MIC=2 μM);
- Gram-negative bacteria : Escherichia coli(KCTC 1682)(MIC=8 μM), Pseudomonas aeruginosa(KCTC 1637)(MIC=4 μM), Salmonella typhimurium(KCTC 1926)(MIC=4 μM)
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Hemolytic Activity
-
- [Ref.28161291] HC50=14.0 μM against human red blood cells
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Cytotoxicity
-
- [Ref.28161291] The cell viability of mouse macrophage RAW264.7 induced by FK13-a2 is 104.5%, 101.7%, 100.4%, 77.0%, 58.9% and 15.8% at peptide concentrations of 1.25, 2.5, 5, 10, 20 and 40 μM.
- The cell viablity of human keratinocyte HaCaT induced by FK
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Random coil, alpha helix
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21235.
Physicochemical Information
-
Formula
- C92H146N30O14
Absent Amino Acids
- ACDEFGHMNPQSTY
Common Amino Acids
- R
Mass
- 1896.36
PI
- 12.48
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +6
-
Boman Index
- -4499
Hydrophobicity
- -0.885
Aliphatic Index
- 112.31
Half Life
-
- Mammalian:2.8 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 16500
Absorbance 280nm
- 1375
Polar Residues
- 0
DRAMP21235
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- LL-37-derived membrane-active FK-13 analogs possessing cell selectivity, anti-biofilm activity and synergy with chloramphenicol and anti-inflammatory activity.
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Pubmed ID
- 28161291
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Reference
- Biochim Biophys Acta Biomembr. 2017 May;1859(5):722-733. doi: 10.1016/j.bbamem.2017.01.037.
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Author
- Rajasekaran G, Kim EY, Shin SY