General Information

    • DRAMP ID

    • DRAMP21239

    • Peptide Name

    • FK13-a6 (Derived from FK13)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • WKRWVQRWKRWLR

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.28161291] Gram-positive bacteria : Bacillus subtilis(KCTC 3068)(MIC=8 μM), Staphylococcus epidermidis(KCTC 1917)(MIC=4 μM), Staphylococcus aureus(KCTC1621)(MIC=2 μM);
      • Gram-negative bacteria : Escherichia coli(KCTC 1682)(MIC=4 μM), Pseudomonas aeruginosa(KCTC 1637)(MIC=16 μM), Salmonella typhimurium(KCTC 1926)(MIC=2 μM)

    • Hemolytic Activity

      • [Ref.28161291] HC50=167.5 μM against human red blood cells

    • Cytotoxicity

      • [Ref.28161291] The cell viability of mouse macrophage RAW264.7 induced by FK13-a6 is 91.3%, 94.6%, 103.0%, 96.6%, 61.9% and 28.7% at peptide concentrations of 1.25, 2.5, 5, 10, 20 and 40 μM.
      • The cell viablity of human keratinocyte HaCaT induced by FK13

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21239 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21239.

Physicochemical Information

    • Formula

    • C96H142N32O15

    • Absent Amino Acids

    • ACDEFGHIMNPSTY

    • Common Amino Acids

    • RW

    • Mass

    • 1984.39

    • PI

    • 12.48

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +6

    • Boman Index

    • -5804

    • Hydrophobicity

    • -1.915

    • Aliphatic Index

    • 52.31

    • Half Life

      • Mammalian:2.8 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 22000

    • Absorbance 280nm

    • 1833.33

    • Polar Residues

    • 0

DRAMP21239

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • LL-37-derived membrane-active FK-13 analogs possessing cell selectivity, anti-biofilm activity and synergy with chloramphenicol and anti-inflammatory activity.

    • Reference

    • Biochim Biophys Acta Biomembr. 2017 May;1859(5):722-733. doi: 10.1016/j.bbamem.2017.01.037.

    • Author

    • Rajasekaran G, Kim EY, Shin SY