General Information

    • DRAMP ID

    • DRAMP21246

    • Peptide Name

    • pEM-2 (Derived from the venom of the snake Bothrops asper)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KKWRWWLKALAKK

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.27591703] Gram-positive bacteria : Staphylococcus aureus isolates(MIC=5.3 ± 2.3 mg/L, MBC=6.7 ± 2.3 mg/L), Enterococcus faecalis isolates(MIC=6.7 ± 2.3 mg/L, MBC=8.0 ± 0.0 mg/L);
      • Gram-negative bacteria : Pseudomonas aeruginosa isolates(MIC=4.0 ± 3.5 mg/L, MBC=4.0 ± 3.5 mg/L), Acinetobacter baumannii isolates(MIC=4.0 ± 0.0 mg/L, MBC=5.3 ± 2.3 mg/L)

    • Hemolytic Activity

      • [Ref.27591703] 0% hemolysis at 2 μg/ml , 5% hemolysis at 4 μg/ml , 15% hemolysis at 8 μg/ml , 55% hemolysis at 16 μg/ml , 87% hemolysis at 32 μg/ml , 92% hemolysis at 64 μg/ml against human red blood cells; 0% hemolysis at 16 μg/ml , 2.5% hemolysis at 32 μg/ml , 10% hemolysis at 64 μg/ml against sheep red blood cells

    • Cytotoxicity

      • [Ref.27591703] The cell viability of HEK293 cells induced by PV is 99.1%, 99.1%, 98.3%, 94.8%, 93.9%, 87.5% and 84.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μg/mL within 24h.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21246 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21246.

Physicochemical Information

    • Formula

    • C87H136N24O14

    • Absent Amino Acids

    • CDEFGHIMNPQSTVY

    • Common Amino Acids

    • K

    • Mass

    • 1742.19

    • PI

    • 11.39

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +6

    • Boman Index

    • -2222

    • Hydrophobicity

    • -1.192

    • Aliphatic Index

    • 75.38

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 16500

    • Absorbance 280nm

    • 1375

    • Polar Residues

    • 0

DRAMP21246

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Design and characterization of short hybrid antimicrobial peptides from pEM-2, mastoparan-VT1, and mastoparan-B.

    • Reference

    • Chem Biol Drug Des. 2017 Mar;89(3):327-338. doi: 10.1111/cbdd.12864.

    • Author

    • Memariani H, Shahbazzadeh D, Ranjbar R, Behdani M, Memariani M, Pooshang Bagheri K