General Information
-
DRAMP ID
- DRAMP21247
-
Peptide Name
- PV (Derived from pEM-2 and MP-VT1)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- KKWRWWLKALAKKLL
-
Sequence Length
- 15
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.27591703] Gram-positive bacteria : Staphylococcus aureus isolates(MIC=0.5 ± 0.0 mg/L, MBC=0.5 ± 0.0 mg/L), Enterococcus faecalis isolates(MIC=0.7 ± 0.3 mg/L, MBC=0.8 ± 0.3 mg/L);
- Gram-negative bacteria : Pseudomonas aeruginosa isolates(MIC=1.2 ± 0.8 mg/L, MBC=1.3 ± 0.6 mg/L), Acinetobacter baumannii isolates(MIC=0.4 ± 0.1 mg/L, MBC=0.5 ± 0.0 mg/L)
-
Hemolytic Activity
-
- [Ref.27591703] 0% hemolysis at 32 μg/ml , 5% hemolysis at 64 μg/ml against human red blood cells; 0% hemolysis at 32 μg/ml , 8% hemolysis at 64 μg/ml against sheep red blood cells
-
Cytotoxicity
-
- [Ref.27591703] The cell viability of HEK293 cells induced by PV is 98.8%, 97.4%, 85.8%, 56.2%, 22.8%, 12.2% and 2.3% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μg/mL within 24h.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21247.
Physicochemical Information
-
Formula
- C99H158N26O16
Absent Amino Acids
- CDEFGHIMNPQSTVY
Common Amino Acids
- K
Mass
- 1968.51
PI
- 11.39
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 9
Net Charge
- +6
-
Boman Index
- -1238
Hydrophobicity
- -0.527
Aliphatic Index
- 117.33
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 16500
Absorbance 280nm
- 1178.57
Polar Residues
- 0
DRAMP21247
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- Design and characterization of short hybrid antimicrobial peptides from pEM-2, mastoparan-VT1, and mastoparan-B.
-
Pubmed ID
- 27591703
-
Reference
- Chem Biol Drug Des. 2017 Mar;89(3):327-338. doi: 10.1111/cbdd.12864.
-
Author
- Memariani H, Shahbazzadeh D, Ranjbar R, Behdani M, Memariani M, Pooshang Bagheri K