General Information

    • DRAMP ID

    • DRAMP21248

    • Peptide Name

    • BVP (Derived from pEM-2 and MP-VT1 and MP-B)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • LKLKAIAALAKKKW

    • Sequence Length

    • 14

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.27591703] Gram-positive bacteria : Staphylococcus aureus isolates(MIC=24.0 ± 13.9 mg/L, MBC=37.3 ± 24.4 mg/L), Enterococcus faecalis isolates(MIC=12.0 ± 6.9 mg/L, MBC=18.7 ± 12.2 mg/L);
      • Gram-negative bacteria : Pseudomonas aeruginosa isolates(MIC=16.0 ± 13.9 mg/L, MBC=18.7 ± 12.2 mg/L), Acinetobacter baumannii isolates(MIC=8.0 ± 0.0 mg/L, MBC=10.7 ± 4.6 mg/L)

    • Hemolytic Activity

      • [Ref.27591703] 0% hemolysis at 64 μg/ml against human red blood cells; 0% hemolysis at 16 μg/ml , 2.5% hemolysis at 32 μg/ml , 10% hemolysis at 64 μg/ml against sheep red blood cells

    • Cytotoxicity

      • [Ref.27591703] The cell viability of HEK293 cells induced by PV is 98.9%, 95.4%, 89.7%, 83.4%, 83.1%, 73.7% and 71.5% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μg/mL within 24h.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21248 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21248.

Physicochemical Information

    • Formula

    • C77H136N20O15

    • Absent Amino Acids

    • CDEFGHMNPQRSTVY

    • Common Amino Acids

    • K

    • Mass

    • 1582.05

    • PI

    • 10.6

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +5

    • Boman Index

    • 150

    • Hydrophobicity

    • 0.193

    • Aliphatic Index

    • 140

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 423.08

    • Polar Residues

    • 0

DRAMP21248

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Design and characterization of short hybrid antimicrobial peptides from pEM-2, mastoparan-VT1, and mastoparan-B.

    • Reference

    • Chem Biol Drug Des. 2017 Mar;89(3):327-338. doi: 10.1111/cbdd.12864.

    • Author

    • Memariani H, Shahbazzadeh D, Ranjbar R, Behdani M, Memariani M, Pooshang Bagheri K