General Information
-
DRAMP ID
- DRAMP21250
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Peptide Name
- PV3 (Derived from pEM-2 and MP-VT1)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- KKWRKLLKKLKKLL
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Sequence Length
- 14
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.27591703] Gram-positive bacteria : Staphylococcus aureus isolates(MIC=2.0 ± 1.7 mg/L, MBC=2.3 ± 1.5 mg/L), Enterococcus faecalis isolates(MIC=1.2 ± 0.8 mg/L, MBC=1.3 ± 0.6 mg/L);
- Gram-negative bacteria : Pseudomonas aeruginosa isolates(MIC=1.0 ± 0.0 mg/L, MBC=1.3 ± 0.6 mg/L), Acinetobacter baumannii isolates(MIC=1.0 ± 0.0 mg/L, MBC=1.7 ± 0.6 mg/L)
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Hemolytic Activity
-
- [Ref.27591703] 0% hemolysis at 64 μg/ml against human red blood cells; 10% hemolysis at 64 μg/ml against sheep red blood cells
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Cytotoxicity
-
- [Ref.27591703] The cell viability of HEK293 cells induced by PV is 98.8%, 99.2%, 95.4%, 93.8%, 91.9%, 77.3% and 55.4% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μg/mL within 24h.
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Binding Target
- Not found
Structure Information
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Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
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Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21250.
Physicochemical Information
-
Formula
- C89H163N25O15
Absent Amino Acids
- ACDEFGHIMNPQSTVY
Common Amino Acids
- K
Mass
- 1823.43
PI
- 11.47
Basic Residues
- 8
Acidic Residues
- 0
Hydrophobic Residues
- 6
Net Charge
- +8
-
Boman Index
- -2684
Hydrophobicity
- -0.979
Aliphatic Index
- 139.29
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 423.08
Polar Residues
- 0
DRAMP21250
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
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Title
- Design and characterization of short hybrid antimicrobial peptides from pEM-2, mastoparan-VT1, and mastoparan-B.
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Pubmed ID
- 27591703
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Reference
- Chem Biol Drug Des. 2017 Mar;89(3):327-338. doi: 10.1111/cbdd.12864.
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Author
- Memariani H, Shahbazzadeh D, Ranjbar R, Behdani M, Memariani M, Pooshang Bagheri K