General Information

    • DRAMP ID

    • DRAMP21257

    • Peptide Name

    • WL3 (Derived from CP-1)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • WLSKTAKKLWLSKTAKKLWLSKTAKKL

    • Sequence Length

    • 27

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.29266746] Gram-positive bacteria : Staphylococcus aureus ATCC 29213(MIC=32 μM), Staphylococcus aureus ATCC 43300(MIC=32 μM), Staphylococcus epidermidis ATCC 12228(MIC=32 μM), Bacillus subtilis CMCC 63501(MIC=8 μM);
      • Gram-negative bacteria : Escherichia coli ATCC25922(MIC=2 μM), Escherichia coli UB1005(MIC=2 μM), Pseudomonas aeruginosa ATCC 27853(MIC=2 μM), Salmonella typhimurium ATCC 7731(MIC=8 μM), Salmonella pullorum C79-13(MIC=2 μM)

    • Hemolytic Activity

      • [Ref.29266746] MHC5>128 μM against human red blood cells

    • Cytotoxicity

      • [Ref.29266746] The cell viability of IPEC-J2 cells induced by WL3 is 97.0%, 100.4%, 91.4%, 91.5%, 87.2%, 86.7% and 78.7% at peptide concentrations of 2, 4, 8, 16, 32, 64 and 128 μM. The cell viability of IPEC-J2 cells induced by the control Melittin is

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21257 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21257.

Physicochemical Information

    • Formula

    • C153H257N39O34

    • Absent Amino Acids

    • CDEFGHIMNPQRVY

    • Common Amino Acids

    • K

    • Mass

    • 3186.96

    • PI

    • 10.9

    • Basic Residues

    • 9

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +9

    • Boman Index

    • -2592

    • Hydrophobicity

    • -0.522

    • Aliphatic Index

    • 97.78

    • Half Life

      • Mammalian:2.8 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 16500

    • Absorbance 280nm

    • 634.62

    • Polar Residues

    • 6

DRAMP21257

DRAMP21257 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Antibacterial activities and molecular mechanism of amino-terminal fragments from pig nematode antimicrobial peptide CP-1.

    • Reference

    • Chem Biol Drug Des. 2018 May;91(5):1017-1029. doi: 10.1111/cbdd.13165.

    • Author

    • Dong N, Wang Z, Chou S, Zhang L, Shan A, Jiang J