General Information
-
DRAMP ID
- DRAMP21262
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Peptide Name
- KL6A4 (De novo synthesis)
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Source
- Synthetic construct
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Family
- Not found
-
Gene
- Not found
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Sequence
- AAKLLLKLLLKAA
-
Sequence Length
- 13
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.26385363] Gram-positive bacteria : Staphylococcus aureus ATCC 29213(MIC=32 μM), Staphylococcus epidermidis ATCC 12228(MIC=16 μM), Streptococcus faecalis ATCC 29212(MIC=16 μM), Bacillus subtilis CMCC 63501(MIC=16 μM);
- Gram-negative bacteria : Escherichia coli ATCC25922(MIC=1 μM), Escherichia coli K88(MIC=16 μM), Escherichia coli CVCC 1515(MIC=16 μM), Escherichia coli UB1005(MIC=32 μM), Bacterium pyocyaneum ATCC 27853(MIC>128 μM), Salmonella typhimurium ATCC 14028(MIC>128 μM), Salmonella pullorum C79–13(MIC>128 μM)
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Hemolytic Activity
-
- [Ref.26385363] MHC5=8 μM against human red blood cells
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Cytotoxicity
-
- [Ref.26385363] The cell viability of HEK293 cells induced by KL6A4 is 102.8%, 109.4%, 103.9%, 109.4%, 105.3%, 90.6%, 83.1% and 81.7% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM. The cell viability of HEK293 cells induced by control Me
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21262.
Physicochemical Information
-
Formula
- C66H124N16O14
Absent Amino Acids
- CDEFGHIMNPQRSTVWY
Common Amino Acids
- L
Mass
- 1365.81
PI
- 10.3
Basic Residues
- 3
Acidic Residues
- 0
Hydrophobic Residues
- 10
Net Charge
- +3
-
Boman Index
- 2011
Hydrophobicity
- 1.408
Aliphatic Index
- 210.77
Half Life
-
- Mammalian:4.4 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 0
DRAMP21262
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
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Title
- Rational design of mirror-like peptides with alanine regulation.
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Pubmed ID
- 26385363
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Reference
- Amino Acids. 2016 Feb;48(2):403-17. doi: 10.1007/s00726-015-2094-y.
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Author
- Li W, Tan T, Xu W, Xu L, Dong N, Ma D, Shan A