• DRAMP ID

    • DRAMP21310
    • Peptide Name

    • RK12 (Derived from PMAP-36)
    • Source

    • synthetic construct
    • Family

    • N/A
    • Gene

    • N/A
    • Sequence

    • RRLRKKTRKRLK
    • Sequence Length

    • 12
    • Swiss_Prot Entry

    • No entry found
    • Evidence code

    • N/A
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
    • Target Organism

      • [Ref.31276398] Gram-positive bacteria:Staphylococcus aureus 29213 (MIC>128 μM), Staphylococcus aureus 25923 (MIC>128 μM), Staphylococcus epidermidis 12228 (MIC>128 μM);
      • Gram-negative bacteria: Escherichia coli 25922 (MIC>128 μM), Escherichia coli 1005 (MIC=64 μM), Staphylococcus typhi (MIC>128 μM)
    • Hemolytic Activity

      • [Ref.31276398] MHC>128μM. The minimum hemolytic concentration (MHC) is measured as the lowest concentration of the peptides that caused 5% hemolysis of human red blood cells (hRBCs).
    • Cytotoxicity

      • [Ref.31276398] The cell viability of the murine macrophage cell line RAW264.7 induced by RK12 is 91.7%, 96.2%, 95.2%, 95.3%, 96.2%, 94.7%, 98.8% and 99.3% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM
    • Binding Target

    • N/A
    • Linear/Cyclic/Branched

    • Linear
    • N-terminal modification

    • Free
    • C-terminal modification

    • Amidation (Lys12)
    • Other modifications

    • None
    • Stereochemistry

    • L
    • Structure

    • No α-helix content in 50%TFE
    • Structure Description

    • N/A
    • DRAMP21310 helical wheel diagram
    • Formula

    • C70H139N31O14
    • Absent Amino Acids

    • ACDEFGHIMNPQSVWY
    • Common Amino Acids

    • R
    • Mass

    • 1639.07
    • PI

    • 12.61
    • Basic Residues

    • 9
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 2
    • Boman Index

    • -8953
    • Hydrophobicity

    • -260
    • Aliphatic Index

    • 65
    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 1

DRAMP21310

    • Function

    • Antimicrobial activity against Gram-positive bacteria and Gram-negative bacteria.
  • Literature 1
    • Reference

    • J Med Chem. 2019 Aug 8;62(15):6941-6957. doi: 10.1021/acs.jmedchem.9b00288.
    • Author

    • Lyu Y, Chen T, Shang L, Yang Y, Li Z, Zhu J, Shan A.
    • Title

    • Design of Trp-rich dodecapeptides with broad-spectrum antimicrobial potency and membrane-disruptive mechanism