General Information
-
DRAMP ID
- DRAMP21321
-
Peptide Name
- 4W (Derived from PMAP-36)
-
Source
- synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- RWLRWWTRWRLK
-
Sequence Length
- 12
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.31276398] Gram-positive bacteria:Staphylococcus aureus 29213 (MIC=4 μM), Staphylococcus aureus 25923 (MIC=2 μM), Staphylococcus epidermidis 12228 (MIC=4 μM);
- Gram-negative bacteria: Escherichia coli 25922 (MIC=4 μM), Escherichia coli 1005 (MIC=2 μM), Staphylococcus typhi (MIC=4 μM)
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Hemolytic Activity
-
- [Ref.31276398] MHC=128μM. The minimum hemolytic concentration (MHC) is measured as the lowest concentration of the peptides that caused 5% hemolysis of human red blood cells (hRBCs).
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Cytotoxicity
-
- [Ref.31276398] The cell viability of the murine macrophage cell line RAW264.7 induced by 4W is 95.3%, 88.3%, 89.7%, 97.9%, 91.2%, 77.6%, 32.8% and 0% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- α-helix in 50%TFE
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21321.
Physicochemical Information
-
Formula
- C90H131N29O14
Absent Amino Acids
- ACDEFGHIMNPQSVY
Common Amino Acids
- RW
Mass
- 1843.22
PI
- 12.48
Basic Residues
- 5
Acidic Residues
- 0
Hydrophobic Residues
- 6
Net Charge
- +5
-
Boman Index
- -4864
Hydrophobicity
- -1.55
Aliphatic Index
- 65
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 22000
Absorbance 280nm
- 2000
Polar Residues
- 1
DRAMP21321
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria.
Literature Information
- ·Literature 1
-
Title
- Design of Trp-rich dodecapeptides with broad-spectrum antimicrobial potency and membrane-disruptive mechanism
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Pubmed ID
- 31276398
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Reference
- J Med Chem. 2019 Aug 8;62(15):6941-6957. doi: 10.1021/acs.jmedchem.9b00299.
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Author
- Lyu Y, Chen T, Shang L, Yang Y, Li Z, Zhu J, Shan A.