• • DRAMP ID

  • DRAMP21334

• • Peptide Name

  • Ranatuerin-2Pb (Frogs, amphibians, animals)

• • Source

  • Lithobates pipiens (Northern leopard frog) (Rana pipiens)

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • SFLTTVKKLVTNLAALAGTVIDTIKCKVTGGCRT

• • Sequence Length

  • 34

• • UniProt Entry

  • A0A513ZTP5

• • Protein Existence

  • Transcript level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.31242693] Gram-positive bacteria:Staphylococcus aureus (MIC=8 μM; MBC=8 μM), MRSA (MIC=16 μM; MBC=32 μM), Enterococcus faecalis (MIC>256 μM; MBC>256 μM);
    • Gram-negative bacteria: Escherichia coli (MIC=8 μM; MBC=8 μM), Pseudomonas aeruginosa (MIC>256 μM; MBC>256 μM);
    • Fungi: Candida albicans (MIC=8 μM; MBC=16 μM)

• • Hemolytic Activity

  • • [Ref.31242693] 7% hemolysis at 2 μM, 23% hemolysis at 8 μM, 50% hemolysis at 16 μM, 71% hemolysis at 32 μM, 100% hemolysis at 64 μM on horse erythrocytes.

• • Cytotoxicity

  • • [Ref.31242693] The IC50 values which represents the inhibitory effect of Ranatuerin-2Pb on the proliferation of NCI-H157, MCF-7, U251MG and PC-3 are 1.453 μM, 7.254 μM, 2.172 μM and 2.251 μM. In addtion, Ranatuerin-2Pb showed no inhibitory effect on the proliferation of MDA-MB-435s. DETAILED DATA: ①The cell viabillity of NCI-H157 induced by Ranatuerin-2Pb is 96.2%, 96.9%, 85.6%, 60%, 5% and 4.4% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 100%, 95%, 89.4%, 41.2%, 16.2% and 16.2% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ②The cell viability of MCF-7 induced by Ranatuerin-2Pb is 100.6%, 92.5%, 90%, 99.4%, 36.2% and 11.2% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 100%, 98.1%, 92.5%, 7.5%, 8.7% and 8.7% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ③The cell viability of U251-MG induced by Ranatuerin-2Pb is 100%, 105.6%, 108.1%, 102.5%, 59.4% and 17.5% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 99.4%, 99.4%, 75.6%, 18.7%, 11.9% and 18.1% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ④The cell viability of PC-3 induced by Ranatuerin-2Pb is 108.7%, 112.5%, 84.4%, 80.6%, 28.1% and 16.2% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 110%, 113.1%, 109.4%, 33.1%, 16.9% and 16.2% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ⑤ The cell viability of MDA-MB-435S induced by Ranatuerin-2Pb is 96.9%, 88.8%, 96.9%, 88.1%, 100 and 25.6%, while the cell viability induced by Melittin is 100%, 93.1%, 82.5%, 18.8%, 18.8% and 18.1%.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • 50% Helix, 7.8% antiparallel, 1.2% parallel, 10.9% turn and 29.8% others in 50% TFE/H2O. ##3.5% Helix, 26.6% antiparallel, 0% parallel, 18.3% turn and

• • Structure Description

  • Ranatuerin-2Pb might also possess two helical segments at respective N-terminus and C-terminus, and the last two residues are probably included in the helix domain.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP21334.

DRAMP21334

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans.

• ·Literature 1

• • Title

  • Bioevaluation of Ranatuerin-2Pb from the Frog Skin Secretion of Rana pipiens and its Truncated Analogues

• • Pubmed ID

  • 31242693

• • Reference

  • Biomolecules. 2019 Jun 25;9(6):249. doi: 10.3390/biom9060249.

• • Author

  • Zhou, XW; Shi, DN; Zhong, RM; Ye, ZM; Ma, CB; Zhou, M; Xi, XP; Wang, L; Chen, TB; Kwok, HF