General Information
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DRAMP ID
- DRAMP21335
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Peptide Name
- RPa (Frogs, amphibians, animals)
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Source
- synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- SFLTTVKKLVTNLAALAGTVIDTIKCKVTGGC
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Sequence Length
- 32
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
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- [Ref.31242693] Gram-positive bacteria:Staphylococcus aureus (MIC=16 μM; MBC=32 μM), MRSA (MIC=>256 μM; MBC>256 μM), Enterococcus faecalis (MIC>256 μM; MBC>256 μM);
- Gram-negative bacteria: Escherichia coli (MIC=32 μM; MBC=64 μM), Pseudomonas aeruginosa (MIC>256 μM; MBC>256 μM);
- Fungi: Candida albicans (MIC>256 μM; MBC>256 μM)
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Hemolytic Activity
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- [Ref.31242693] 7% hemolysis at 16 μM, 23% hemolysis at 32 μM, 50% hemolysis at 64 μM, 76% hemolysis at 128 μM, 90% hemolysis at 256 μM, 100% hemolysis at 512 μM on horse erythrocytes.
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Cytotoxicity
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- [Ref.31242693] RPa only possessed inhibitory effect on the proliferation of H157 with the IC value of 5.841 μM, while showed no inhibitory effect on the proliferation of MCF-7, U251MG, PC-3 and MDA-MB-435s. DETAILED DATA: ①The cell viabillity of NCI-H157 induced by RPa is 97.5%, 94.4%, 92.5%, 71.2%, 40.6% and 4.4% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 100%, 95%, 89.4%, 41.2%, 16.2% and 16.2% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ②The cell viability of MCF-7 induced by RPa is 100.6%, 93.1%, 97.5%, 97.5%, 93.7% and 78.1% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM , while the cell viability induced by Melittin is 100%, 98.1%, 92.5%, 7.5%, 8.7% and 8.7% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ③The cell viability of U251-MG induced by RPa is 100%, 105.6%, 108.1%, 102.5%, 59.4% and 17.5% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 99.4%, 99.4%, 75.6%, 18.7%, 11.9% and 18.1% at 0.001, 0.01, 0.1, 1, 10, 100 μM.④The cell viability of PC-3 induced by RPb is 108.7%, 104.4%, 92.5%, 92.5%, 82.5% and 24.4% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 110%, 113.1%, 109.4%, 33.1%, 16.9% and 16.2% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ⑤The cell viability of MDA-MB-435S induced by RPa is 97.5%, 95%, 97.5%, 73.1%, 68.8% and 36.9%, while the cell viability induced by Melittin is 100%, 93.1%, 82.5%, 18.8%, 18.8% and 18.1%.
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Binding Target
- Not found
Structure Information
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Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Free
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
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Structure
- 33.6% Helix, 18.1% antiparallel, 4.3% parallel, 11.4% turn and 32.5% others in 50% TFE/H2O. ##2.4% Helix, 30.6% antiparallel, 0% parallel, 17.3% turn
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Structure Description
- Not found
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21335.
Physicochemical Information
-
Formula
- C145H253N37O43S2
Absent Amino Acids
- EHMPQRWY
Common Amino Acids
- T
Mass
- 3266.95
PI
- 9.3
Basic Residues
- 4
Acidic Residues
- 1
Hydrophobic Residues
- 14
Net Charge
- +3
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Boman Index
- 309
Hydrophobicity
- 0.794
Aliphatic Index
- 118.75
Half Life
-
- Mammalian:1.9 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 4.03
Polar Residues
- 13
DRAMP21335
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans.
Literature Information
- ·Literature 1
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Title
- Bioevaluation of Ranatuerin-2Pb from the Frog Skin Secretion of Rana pipiens and its Truncated Analogues
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Pubmed ID
- 31242693
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Reference
- Biomolecules. 2019 Jun 25;9(6):249. doi: 10.3390/biom9060249.
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Author
- Zhou, XW; Shi, DN; Zhong, RM; Ye, ZM; Ma, CB; Zhou, M; Xi, XP; Wang, L; Chen, TB; Kwok, HF