General Information

    • DRAMP ID

    • DRAMP21337

    • Peptide Name

    • BMAP-27 (Bovine, mammals, animals)

    • Source

    • Bos taurus (Bovine)

    • Family

    • Belongs to the cathelicidin family

    • Gene

    • CATHL6

    • Sequence

    • GRFKRFRKKFKKLFKKLSPVIPLLHL

    • Sequence Length

    • 26

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.31226350] Gram-positive bacteria:Staphylococcus aureus (MIC=1-2 μM);
      • Gram-negative bacteria: Escherichia coli (MIC=2-4 μM)

    • Hemolytic Activity

      • [Ref.31226350] 50% hemolysis at 75 μM for human red blood cells

    • Cytotoxicity

      • [Ref.31226350] The CC50 values which represent peptide concentration (μM) inducing 50% cytotoxicity to human cancer cell lines of BMAP-27 on MDA361 and A549 are 4.2 μM and 5.3 μM. DETAILED DATA: ①The cell viability of MDA-361 induced by BMAP-27 is 100%, 100%, 98.9%, 97.7%, 87.6%, 59.8%, 34.7%, 12.4%, 10.3%, 8.3% and 9.4% at peptide concentrations of 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4, 13, 25, 50 and 100 μM. ②The cell viability of A549 induced by BMAP-27 is 100%, 99.1%, 97.7%, 94.7%, 74.5%, 41.4%, 24.4%, 11.3%, 9.7%, 10.3% and 7.4% at peptide concentrations of 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4, 13, 25, 50 and 100 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • 73% α-helix in 50% TFE/H2O

    • Structure Description

    • An α-helix occurred from Arg2 to Leu17 in the N-terminal region and a short α-helix is observed from Leu23 to Leu26 in the Cterminal region; the two regions are connected with a flexible region containing two proline residues.

    • Helical Wheel Diagram

    • DRAMP21337 helical wheel diagram

    • PDB ID

    • 2KET resolved by NMR

    • Predicted Structure

    • There is no predicted structure for DRAMP21337.

Physicochemical Information

    • Formula

    • C158H262N44O28

    • Absent Amino Acids

    • ACDEMNQTWY

    • Common Amino Acids

    • K

    • Mass

    • 3226.1

    • PI

    • 12.32

    • Basic Residues

    • 11

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +11

    • Boman Index

    • -4525

    • Hydrophobicity

    • -0.365

    • Aliphatic Index

    • 101.15

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP21337

DRAMP21337 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Structural analysis and mode of action of BMAP-27, a cathelicidin-derived antimicrobial peptide

    • Reference

    • Peptides. 2019 Jun 18;118:170106. doi: 10.1016/j.peptides.2019.170106.

    • Author

    • Yang S, Lee CW, Kim HJ, Jung HH, Kim JI, Shin SY, Shin SH