General Information
-
DRAMP ID
- DRAMP21341
-
Peptide Name
- [Gly]1-VmCT1-NH2 (Derived from VmCT1)
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Source
- synthetic construct
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Family
- Not found
-
Gene
- Not found
-
Sequence
- GLGALWNVAKSVF
-
Sequence Length
- 13
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.31212183] Gram-positive bacteria: Micrococcus luteus A270 (MIC=0.8 μmol/L), Staphylococcus aureus ATCC 29213 (MIC=25 μmol/L), Bacillus megaterium ATCC 10778 (MIC=3.1 μmol/L), Bacillus subtilis ATCC 6633 (MIC=3.1 μmol/L);
- Gram-negative bacteria: Escherichia coli SBS 363 (MIC=50 μmol/L),Serratia marcescens ATCC 4112 (MIC=0.4 μmol/L);
- Fungi: Candida albicans MDM8 (MIC=25 μmol/L), Candida tropicalis IOC 4560 (MIC=6.3 μmol/L)
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Hemolytic Activity
-
- [Ref.31212183] 10% hemolysis at 50 μmol/L, 30% hemolysis at 100 μmol/L for human red blood cells
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- 37% α-helix in 60% TFE/Water. ## 2%α-helix in water.
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21341.
Physicochemical Information
-
Formula
- C65H100N16O16
Absent Amino Acids
- CDEHIMPQRTY
Common Amino Acids
- AGLV
Mass
- 1361.61
PI
- 8.75
Basic Residues
- 1
Acidic Residues
- 0
Hydrophobic Residues
- 8
Net Charge
- +1
-
Boman Index
- 1314
Hydrophobicity
- 0.962
Aliphatic Index
- 120
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 458.33
Polar Residues
- 4
DRAMP21341
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans and Candida tropicalis IOC 4563.
Literature Information
- ·Literature 1
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Title
- Repurposing the scorpion venom peptide VmCT1 into an active peptide against Gram-negative ESKAPE pathogens
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Pubmed ID
- 31212183
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Reference
- Bioorg Chem. 2019 Jun 8;90:103038. doi: 10.1016/j.bioorg.2019.103038.
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Author
- Pedron CN, Araújo I, da Silva Junior PI, Dias da Silva F, Torres MT, Oliveira Junior VX