General Information

    • DRAMP ID

    • DRAMP21356

    • Peptide Name

    • 4Kamp (De Novo Synthesis)

    • Source

    • synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KKKKAAFAKAWAKAFAA

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.31194548] Gram-negative Bacteria: Escherichia coli K12 (MIC=12.5μM), Pseudomonas aeruginosa PAO1(MIC=50μM), Pseudomonas aeruginosa PA214(MIC=50μM), Pseudomonas aeruginosa PA287(MIC>50μM), Pseudomonas aeruginosa PA330(MIC>50μM), Pseudomonas aeruginosa PA380(MIC=50μM)

    • Hemolytic Activity

      • [Ref.31194548] 0% hemolysis at 40μM against human blood cells.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helix mainly in 10 mM Tris buffer 10 Mm NaCl pH 7.4, supplemented with either 2 mg/ml E.coli or POPC lipid extract.

    • Structure Description

    • Peptides are shown as ideal helices with side chains shown as stick models for Lys, Trp, and Phe and as space-filling models.

    • Helical Wheel Diagram

    • DRAMP21356 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21356.

Physicochemical Information

    • Formula

    • C89H142N24O18

    • Absent Amino Acids

    • CDEGHILMNPQRSTVY

    • Common Amino Acids

    • A

    • Mass

    • 1836.26

    • PI

    • 10.7

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +6

    • Boman Index

    • -1053

    • Hydrophobicity

    • -0.253

    • Aliphatic Index

    • 47.06

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 343.75

    • Polar Residues

    • 0

DRAMP21356

Comments Information

    • Function

    • Antibacterial activity against Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Positive Charge Patterning and Hydrophobicity of Membrane-Active Antimicrobial Peptides as Determinants of Activity, Toxicity, and Pharmacokinetic Stability

    • Reference

    • J Med Chem. 2019 Jul 1. doi: 10.1021/acs.jmedchem.9b00657.

    • Author

    • Stone TA, Cole GB, Ravamehr-Lake D, Nguyen HQ, Khan F, Sharpe S, Deber CM