General Information
-
DRAMP ID
- DRAMP21359
-
Peptide Name
- 1Kamp (De Novo Synthesis)
-
Source
- synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- KAAKKFAKAWAKAFKAA
-
Sequence Length
- 17
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
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Target Organism
-
- [Ref.31194548] Gram-negative Bacteria: Escherichia coli K12 (MIC=12.5μM), Pseudomonas aeruginosa PAO1(MIC=50μM), Pseudomonas aeruginosa PA214(MIC>50μM), Pseudomonas aeruginosa PA287(MIC>50μM), Pseudomonas aeruginosa PA330(MIC>50μM), Pseudomonas aeruginosa PA380(MIC>50μM)
-
Hemolytic Activity
-
- [Ref.31194548] 0% hemolysis at 40μM against human blood cells.
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- α-helix mainly in 10 mM Tris buffer 10 Mm NaCl pH 7.4, supplemented with either 2 mg/ml E.coli or POPC lipid extract.
-
Structure Description
- Peptides are shown as ideal helices with side chains shown as stick models for Lys, Trp, and Phe and as space-filling models.
-
Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21359.
Physicochemical Information
-
Formula
- C89H142N24O18
Absent Amino Acids
- CDEGHILMNPQRSTVY
Common Amino Acids
- A
Mass
- 1836.26
PI
- 10.7
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 11
Net Charge
- +6
-
Boman Index
- -1053
Hydrophobicity
- -0.253
Aliphatic Index
- 47.06
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 343.75
Polar Residues
- 0
DRAMP21359
Comments Information
Function
- Antibacterial activity against Gram-negative bacteria.
Literature Information
- ·Literature 1
-
Title
- Positive Charge Patterning and Hydrophobicity of Membrane-Active Antimicrobial Peptides as Determinants of Activity, Toxicity, and Pharmacokinetic Stability
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Pubmed ID
- 31194548
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Reference
- J Med Chem. 2019 Jul 1. doi: 10.1021/acs.jmedchem.9b00657.
-
Author
- Stone TA, Cole GB, Ravamehr-Lake D, Nguyen HQ, Khan F, Sharpe S, Deber CM