General Information

    • DRAMP ID

    • DRAMP21368

    • Peptide Name

    • [Lys]1[Lys]12-VmCT1-NH2 (Derived from VmCT1)

    • Source

    • synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KLGALWNVAKSKF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.31181304] Gram-positive bacteria: Micrococcus luteus A270 (MIC=12.5 μmol/L), Bacillus megaterium ATCC 10778 (MIC=6.3 μmol/L);
      • Gram-negative bacteria: Enterobacter cloacae β-12 (MIC=25μmol/L);
      • Fungi: Candida albicans MDM8 (MIC=6.3 μmol/L), Candida tropicalis IOC 4560 (MIC=3.1 μmol/L)

    • Hemolytic Activity

      • [Ref.31181304] 0% hemolysis at 100 μmol/L against human red blood cells

    • Cytotoxicity

      • [Ref.31181304] The cell viability of MCF-7 cells induced by [Lys1][Lys12]-VmCT1-NH2 is 105%, 110%, 95%, 100%, 110%, 120%, 120% and 120% at peptide concentrations of 0.8, 1.6, 3.1, 12.5, 25, 50 and 100 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • 2% Helical content in water, 0% helical content in PBS (pH7.4), 19% helical content in SDS (20 mmol/L), 13% helical content in TFE/Water (3:2, v:v), 0

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21368 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21368.

Physicochemical Information

    • Formula

    • C70H112N18O16

    • Absent Amino Acids

    • CDEHIMPQRTY

    • Common Amino Acids

    • K

    • Mass

    • 1461.77

    • PI

    • 10.3

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +3

    • Boman Index

    • -294

    • Hydrophobicity

    • 0.069

    • Aliphatic Index

    • 97.69

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 458.33

    • Polar Residues

    • 3

DRAMP21368

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans MDM8 and Candida tropicalis IOC 4560.

Literature Information

  • ·Literature 1

    • Title

    • The effect of lysine substitutions in the biological activities of the scorpion venom peptide VmCT1

    • Reference

    • Eur J Pharm Sci. 2019 Jun 7;136:104952. doi: 10.1016/j.ejps.2019.06.006.

    • Author

    • Pedron CN, de Oliveira CS, da Silva AF, Andrade GP, da Silva Pinhal MA, Cerchiaro G, da Silva Junior PI, da Silva FD, Torres MT, Oliveira VX