• • DRAMP ID

  • DRAMP21369

• • Peptide Name

  • [Lys]3[Lys]7-VmCT1-NH2 (Derived from VmCT1)

• • Source

  • synthetic construct

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • FLKALWKVAKSVF

• • Sequence Length

  • 13

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.31181304] Gram-positive bacteria: Micrococcus luteus A270 (MIC=0.4 μmol/L), Staphylococcus aureus ATCC 29213 (MIC=0.4 μmol/L), Bacillus megaterium ATCC 10778 (MIC=0.4 μmol/L), Staphylococcus epidermidis ATCC 12228 (MIC=3.1 μmol/L);
    • Gram-negative bacteria: Escherichia coli SBS 363 (MIC=1.6 μmol/L)，Serratia marcescens ATCC 4112 (MIC=0.1 μmol/L), Enterobacter cloacae β-12 (MIC=3.1 μmol/L);
    • Fungi: Candida albicans MDM8 (MIC=0.8 μmol/L), Candida tropicalis IOC 4560 (MIC=0.4 μmol/L)

• • Hemolytic Activity

  • • [Ref.31181304] 15% hemolysis at 3.1 μmol/L, 45% hemolysis at 6.3 μmol/L, 65% hemolysis at 12.5 μmol/L, 85% hemolysis at 25 μmol/L, 90% hemolysis at 100 μmol/L against human red blood cells

• • Cytotoxicity

  • • [Ref.31181304] ①The cell viability of MCF-7 cells induced by [Lys3][Lys7]-VmCT1-NH2 is 95%, 95%, 90%, 85%, 80%, 70%, 50%, 40% at peptide concentrations of 0.8, 1.6, 3.1, 12.5, 25, 50 and 100 μM. ②The cell viability of MCF-10A cells induced by [Lys3][Lys7]-VmCT1-NH2 is 120%, 120%, 120%,120%, 95% and 90% at peptide concentrations of 0.8, 1.6, 3.1, 6.3, 12.5 and 25 μM.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • 0% Helical content in water, 5% helical content in PBS (pH7.4), 37% helical content in SDS (20 mmol/L), 52% helical content in TFE/Water (3:1, v:v), 0

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP21369.

DRAMP21369

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans MDM8 and Candida tropicalis IOC 4560.

• ·Literature 1

• • Title

  • The effect of lysine substitutions in the biological activities of the scorpion venom peptide VmCT1

• • Pubmed ID

  • 31181304

• • Reference

  • Eur J Pharm Sci. 2019 Jun 7;136:104952. doi: 10.1016/j.ejps.2019.06.006.

• • Author

  • Pedron CN, de Oliveira CS, da Silva AF, Andrade GP, da Silva Pinhal MA, Cerchiaro G, da Silva Junior PI, da Silva FD, Torres MT, Oliveira VX