General Information
-
DRAMP ID
- DRAMP21372
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Peptide Name
- [Lys]3[Lys]7[Lys]11-VmCT1-NH2 (Derived from VmCT1)
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Source
- synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- FLKALWKVAKKVF
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Sequence Length
- 13
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.31181304] Gram-positive bacteria: Micrococcus luteus A270 (MIC=0.4 μmol/L), Staphylococcus aureus ATCC 29213 (MIC=0.8 μmol/L), Bacillus megaterium ATCC 10778 (MIC=0.2 μmol/L), Staphylococcus epidermidis ATCC 12228 (MIC=3.1 μmol/L);
- Gram-negative bacteria: Escherichia coli SBS 363 (MIC=1.6 μmol/L),Serratia marcescens ATCC 4112 (MIC=0.1μmol/L), Enterobacter cloacae β-12 (MIC=3.1 μmol/L);
- Fungi: Candida albicans MDM8 (MIC=0.8 μmol/L), Candida tropicalis IOC 4560 (MIC=0.4μmol/L)
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Hemolytic Activity
-
- [Ref.31181304] 40% hemolysis at 12.5 μmol/L, 60% hemolysis at 25 μmol/L, 80% hemolysis at 50 μmol/L, 100% hemolysis at 100 μmol/L against human red blood cells
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Cytotoxicity
-
- [Ref.31181304] ①The cell viability of MCF-7 cells induced by [Lys3][Lys7][Lys11]-VmCT1-NH2 is 90%, 90%, 90%, 60%, 50%, 30%, 20% and 20% at peptide concentrations of 0.8, 1.6, 3.1, 12.5, 25, 50 and 100 μM. ②The cell viability of MCF-10A cells induced by [Lys3][Lys7][Lys11]-VmCT1-NH2 is 120%, 120%, 85%, 120%, 115% and 120%at peptide concentrations of 0.8, 1.6, 3.1, 6.3, 12.5 and 25 μM.
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
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Structure
- 0% Helical content in water, 3% helical content in PBS (pH7.4), 25% helical content in SDS (20 mmol/L), 23% helical content in TFE/Water (3:2, v:v), 2
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21372.
Physicochemical Information
-
Formula
- C81H128N18O14
Absent Amino Acids
- CDEGHIMNPQRSTY
Common Amino Acids
- K
Mass
- 1578.02
PI
- 10.48
Basic Residues
- 4
Acidic Residues
- 0
Hydrophobic Residues
- 9
Net Charge
- +4
-
Boman Index
- 763
Hydrophobicity
- 0.669
Aliphatic Index
- 120
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 458.33
Polar Residues
- 0
DRAMP21372
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans MDM8 and Candida tropicalis IOC 4560.
Literature Information
- ·Literature 1
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Title
- The effect of lysine substitutions in the biological activities of the scorpion venom peptide VmCT1
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Pubmed ID
- 31181304
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Reference
- Eur J Pharm Sci. 2019 Jun 7;136:104952. doi: 10.1016/j.ejps.2019.06.006.
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Author
- Pedron CN, de Oliveira CS, da Silva AF, Andrade GP, da Silva Pinhal MA, Cerchiaro G, da Silva Junior PI, da Silva FD, Torres MT, Oliveira VX