General Information

    • DRAMP ID

    • DRAMP21386

    • Peptide Name

    • SP13 (De Novo Synthesis)

    • Source

    • synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • HFKIRKRFVKKLV

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.31163671] Gram-negative bacteria:Escherichia coli ATCC 25922 (MIC >100 µg/mL)

    • Hemolytic Activity

      • [Ref.31163671] 10% hemolysis at >100 μg/mL against human blood cells.

    • Cytotoxicity

      • [Ref.31163671] The cell viability of Hepa 1-6 induced by SP13 is 104.1%, 106.8%, 111.5%, 101.4%, 107.4%, 118.2% at peptide concentrations of 3.125, 6.25, 12.5, 25, 50 and 100 μg/mL.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helix mainly

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21386 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21386.

Physicochemical Information

    • Formula

    • C82H139N25O14

    • Absent Amino Acids

    • ACDEGMNPQSTWY

    • Common Amino Acids

    • K

    • Mass

    • 1699.16

    • PI

    • 12.03

    • Basic Residues

    • 7

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +7

    • Boman Index

    • -3282

    • Hydrophobicity

    • -0.423

    • Aliphatic Index

    • 104.62

    • Half Life

      • Mammalian:3.5 hour
      • Yeast:10 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 0

DRAMP21386

Comments Information

    • Function

    • Antibacterial activity against Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • De Novo Design and In Vitro Testing of Antimicrobial Peptides against Gram-Negative Bacteria.

    • Reference

    • Pharmaceuticals (Basel). 2019 Jun 3;12(2). pii: E82. doi: 10.3390/ph12020082.

    • Author

    • Boris Vishnepolsky, George Zaalishvili, Margarita Karapetian, Tornike Nasrashvili, Nato Kuljanishvili, Andrei Gabrielian, Alex Rosenthal, Darrell E. Hurt, Michael Tartakovsky, Maya Grigolava and Malak Pirtskhalava