General Information

    • DRAMP ID

    • DRAMP21388

    • Peptide Name

    • SP15 * (De Novo Synthesis)

    • Source

    • synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RWIRWVWRKKLR

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.31163671] Gram-negative bacteria:Escherichia coli ATCC 25922 (MIC= 3.125–6.25 µg/mL)

    • Hemolytic Activity

      • [Ref.31163671] 10% hemolysis at >100 μg/mL against human blood cells.

    • Cytotoxicity

      • [Ref.31163671] The cell viability of Hepa 1-6 induced by SP15 is 90.5%, 90.5%, 89.2%, 83.1%, 93.2% and 52.7% at peptide concentrations of 3.125, 6.25, 12.5, 25, 50 and 100 μg/mL.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helix mainly

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21388 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21388.

Physicochemical Information

    • Formula

    • C86H135N29O13

    • Absent Amino Acids

    • ACDEFGHMNPQSTY

    • Common Amino Acids

    • R

    • Mass

    • 1783.2

    • PI

    • 12.48

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +6

    • Boman Index

    • -4991

    • Hydrophobicity

    • -1.333

    • Aliphatic Index

    • 89.17

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 16500

    • Absorbance 280nm

    • 1500

    • Polar Residues

    • 0

DRAMP21388

Comments Information

    • Function

    • Antibacterial activity against Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • De Novo Design and In Vitro Testing of Antimicrobial Peptides against Gram-Negative Bacteria.

    • Reference

    • Pharmaceuticals (Basel). 2019 Jun 3;12(2). pii: E82. doi: 10.3390/ph12020082.

    • Author

    • Boris Vishnepolsky, George Zaalishvili, Margarita Karapetian, Tornike Nasrashvili, Nato Kuljanishvili, Andrei Gabrielian, Alex Rosenthal, Darrell E. Hurt, Michael Tartakovsky, Maya Grigolava and Malak Pirtskhalava