General Information

    • DRAMP ID

    • DRAMP21394

    • Peptide Name

    • K30 (Derived from ATG16)

    • Source

    • synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RWKRHISEQLRRRDRLQRQAJ

    • Sequence Length

    • 21

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.31145591] Gram-positive bacteria: Staphylococcus aureus(MIC=0.9-1.8 μM);
      • Gram-negative bacteria: Escherichia coli (MIC=6.4-12.8μM), Pseudomonas aeruginosa (MIC=13.5-27μM);
      • Fungi: Candida albicans (MIC=30-60μM)

    • Hemolytic Activity

      • [Ref.31145591] 3% hemolysis at 600μM, 4% hemolysis at 700 μM, 5% hemolysis at 800 μM, 6% hemolysis at 900 μM, 9% hemolysis at 1000 μM,against mouse blood cells.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Acylation (Arg1 conjugated with 4-methylhexanoyl group)

    • C-terminal Modification

    • Amidation (2-Nal, position 21)

    • Nonterminal Modifications and Unusual Amino Acids

    • The 'J' in the sequence is the 2-naphthyl alanine (2-Nal) residue.

    • Stereochemistry

    • L

    • Structure

    • Helix–loop–helix in the presence of anionic membrane lipids mimicking bacterial membranes.

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21394 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21394.

Physicochemical Information

    • Formula

    • C113H192N48O28

    • Absent Amino Acids

    • CFGMNPTVY

    • Common Amino Acids

    • R

    • Mass

    • 2802.25

    • PI

    • 12.13

    • Basic Residues

    • 9

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +6

    • Boman Index

    • -13130

    • Hydrophobicity

    • -2.091

    • Aliphatic Index

    • 60.48

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 275

    • Polar Residues

    • 1

DRAMP21394

DRAMP21394 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans.

Literature Information

  • ·Literature 1

    • Title

    • Design, Synthesis, Antibacterial Potential, and Structural Characterization of N-Acylated Derivatives of the Human Autophagy 16 Polypeptide.

    • Reference

    • Bioconjug Chem. 2019 Jun 18. doi: 10.1021/acs.bioconjchem.9b00290.

    • Author

    • Varnava KG, Mohid SA, Calligari P, Stella L, Reynison J, Bhunia A, Sarojini V