General Information
-
DRAMP ID
- DRAMP21396
-
Peptide Name
- K33 (Derived from ATG16)
-
Source
- synthetic construct
-
Family
- Not found
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Gene
- Not found
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Sequence
- RWKRHISEQLRRRDRLQRQAJ
-
Sequence Length
- 21
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.31145591] Gram-positive bacteria: Staphylococcus aureus(MIC=1.8-3.2 μM);
- Gram-negative bacteria: Escherichia coli (MIC=3.2-6.4μM), Pseudomonas aeruginosa (MIC=6.4-12.8μM);
- Fungi: Candida albicans (MIC=1.8-3.7μM)
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Hemolytic Activity
-
- [Ref.31145591] 75% hemolysis at 10μM, 97% hemolysis at 123μM, 100% hemolysis at 500μM against mouse blood cells.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Acylation (Arg1 conjugated with palmitoyl group)
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C-terminal Modification
- Amidation (2-Nal, position 21)
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Nonterminal Modifications and Unusual Amino Acids
- The 'J' in the sequence is the 2-naphthyl alanine (2-Nal) residue.
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Stereochemistry
- L
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Structure
- Unknown
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21396.
Physicochemical Information
-
Formula
- C113H192N48O28
Absent Amino Acids
- CFGMNPTVY
Common Amino Acids
- R
Mass
- 2802.25
PI
- 12.13
Basic Residues
- 9
Acidic Residues
- 2
Hydrophobic Residues
- 5
Net Charge
- +6
-
Boman Index
- -13130
Hydrophobicity
- -2.091
Aliphatic Index
- 60.48
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 275
Polar Residues
- 1
DRAMP21396
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans.
Literature Information
- ·Literature 1
-
Title
- Design, Synthesis, Antibacterial Potential, and Structural Characterization of N-Acylated Derivatives of the Human Autophagy 16 Polypeptide.
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Pubmed ID
- 31145591
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Reference
- Bioconjug Chem. 2019 Jun 18. doi: 10.1021/acs.bioconjchem.9b00290.
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Author
- Varnava KG, Mohid SA, Calligari P, Stella L, Reynison J, Bhunia A, Sarojini V