General Information

    • DRAMP ID

    • DRAMP21399

    • Peptide Name

    • Pep-H (Human, mammals, animals)

    • Source

    • Homo sapiens (Human)

    • Family

    • Belongs to the alpha-defensin family.

    • Gene

    • Not found

    • Sequence

    • RRYGTCIYQGRLWAF

    • Sequence Length

    • 15

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • [Ref.31133658] Gram-positive bacteria:M.tb H37Rv (MIC=10 μg/ml)

    • Hemolytic Activity

      • [Ref.31133658] 2.7% hemolysis at 1μg/ml, 5% hemolysis at 5μg/ml, 6% hemolysis at 50μg/ml, 8% hemolysis at 100 μg/ml against human blood cells.

    • Cytotoxicity

      • [Ref.31133658] The cell viability of MDMs induced by Pep-H is 94.2%, 91.5%, 90.4%, 88.0% and 84.1% at peptide concentrations of 1, 5, 25, 50, 100 μg/mL

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • β-strand mainly (according to PDB entry 3HJ2)

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21399 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21399.

Physicochemical Information

    • Formula

    • C87H128N26O20S

    • Absent Amino Acids

    • DEHKMNPSV

    • Common Amino Acids

    • R

    • Mass

    • 1890.2

    • PI

    • 10.05

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +3

    • Boman Index

    • -3303

    • Hydrophobicity

    • -0.44

    • Aliphatic Index

    • 58.67

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 8480

    • Absorbance 280nm

    • 605.71

    • Polar Residues

    • 6

DRAMP21399

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Exploiting chitosan and gold nanoparticles for antimycobacterial activity of in silico identified antimicrobial motif of human neutrophil peptide-1

    • Reference

    • Sci Rep. 2019; 9: 7866. Published online 2019 May 27. doi: 10.1038/s41598-019-44256-6

    • Author

    • Richa Sharma， Ragini Raghav, Kumari Priyanka, Praveen Rishi, Sadhna Sharma, Sudha Srivastava, Indu Verma