General Information

    • DRAMP ID

    • DRAMP21401

    • Peptide Name

    • NBC2254 (De Novo Synthesis) 

    • Source

    • synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • CALKLTKAKRLVRKIGF

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.31119600] Gram-positive bacteria:MRSA(MIC=73 ± 0.02µM);
      • Gram-negative bacteria:Escherichia coli O157:H7(MIC=3.5 ± 0.05µM)

    • Hemolytic Activity

      • [Ref.31119600] No hemolytic activity against bovine blood cells

    • Cytotoxicity

      • [Ref.31119600] The cell viability of Vero ATCC CCL 1-81 cells induced by NBC2254 is 93.7%, 90.1%, 89.5%, 95.9% and 78.6% at peptide concentrations of 1.2, 3.7, 11.1, 33.3 and 100 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • The sulfhydryl side chain of the cysteine residue was conjugated to AuNC(previously modified with SPDP) via a disulfide bond.

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21401 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21401.

Physicochemical Information

    • Formula

    • C89H161N27O19S

    • Absent Amino Acids

    • DEHMNPQSWY

    • Common Amino Acids

    • K

    • Mass

    • 1945.49

    • PI

    • 11.17

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +6

    • Boman Index

    • -2207

    • Hydrophobicity

    • 0.194

    • Aliphatic Index

    • 120.59

    • Half Life

      • Mammalian:1.2 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP21401

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.

Literature Information

  • ·Literature 1

    • Title

    • New Synthetic Peptides Conjugated to Gold Nanoclusters: Antibiotic Activity Against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA).

    • Reference

    • Protein J. 2019 May 22. doi: 10.1007/s10930-019-09840-9.

    • Author

    • Prada YA, Guzmán F, Ortíz C, Cabanzo R, Torres R, Mejía-Ospino E