General Information

    • DRAMP ID

    • DRAMP21402

    • Peptide Name

    • B1 (Derived from LL-37 and BMAP-27)

    • Source

    • synthetic construct

    • Family

    • Belongs to the cathelicidin family.

    • Gene

    • Not found

    • Sequence

    • KFKKLFKKLSPVFKRIVQRIKDFLR

    • Sequence Length

    • 25

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.31118709] Gram-positive bacteria:Staphylococcus aureus ATCC 29213(MIC=20μM), Staphylococcus aureus ATCC 33591(MIC=20μM), Staphylococcus aureus ATCC 43300(MIC=20μM), Staphylococcus aureus ATCC BAA-41(MIC=20μM);
      • Gram-negative bacteria: Escherichia coli 25922 (MIC=20μM), Escherichia coli 35218 (MIC=20μM), Pseudomonas aeruginosa BAA-2114 (MIC=10μM)

    • Hemolytic Activity

      • [Ref.31118709] 0% hemolysis at 10μM, 8% hemolysis at 25μM, 19% hemolysis at 50μM, 23% hemolysis at 75μM, 30% hemolysis at 100μM, 38% hemolysis at 125μM, 40% hemolysis at 150μM, 43% hemolysis at 175μM, 50% hemolysis at 200μM against human blood cells.

    • Cytotoxicity

      • [Ref.31118709] The cell viability of HEK293 mammalian cell line induced by B1 is 87.0%, 67.1%, 52.3%, 54.3%, 50.6% and 50% at peptide concentrations of 10, 20, 40, 60, 80 and 100 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • 88 % of the α-helices and 12% of the random coil was predicted using HNN

    • Structure Description

    • The helicity percentages of the secondary structure of B1 are predicted using HNN

    • Helical Wheel Diagram

    • DRAMP21402 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21402.

Physicochemical Information

    • Formula

    • C153H256N42O30

    • Absent Amino Acids

    • ACEGHMNTWY

    • Common Amino Acids

    • K

    • Mass

    • 3163.98

    • PI

    • 11.77

    • Basic Residues

    • 10

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +9

    • Boman Index

    • -5667

    • Hydrophobicity

    • -0.408

    • Aliphatic Index

    • 101.2

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 1

DRAMP21402

DRAMP21402 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Design and characterization of a new hybrid peptide from LL-37 and BMAP-27

    • Reference

    • Infect Drug Resist. 2019; 12: 1035–1045. Published online 2019 Apr 30. doi: 10.2147/IDR.S199473

    • Author

    • Yara Al Tall, Ahmad Abualhaijaa, Mohammad Alsaggar, Ammar Almaaytah, Majed Masadeh, and Karem H Alzoub