• • DRAMP ID

  • DRAMP21404

• • Peptide Name

  • peptide 2 (De Novo Synthesis)

• • Source

  • synthetic construct

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • ITKVITKLLNRLTKILSK

• • Sequence Length

  • 18

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.31117348] Gram-positive bacteria:Staphylococcus aureus ATCC 6538(MIC=15 ± 2.5μM), Bacillus subtilis ATCC 6633(MIC=15 ± 2.5μM);
    • Gram-negative bacteria:Escherichia coli ATCC 15597(MIC=3.1μM), Pseudomonas aeruginosa (MIC=25μM), Salmonella Typhimurium 6192(MIC=40 ± 5μM), Klebsiella pneumoniae NCTC 5055(MIC=50μM)

• • Hemolytic Activity

  • • [Ref.31117348] 5% hemolysis at 250 μM against human blood cells.

• • Cytotoxicity

  • • [Ref.31117348] ①The cell viability of MCF7 cells induced by peptide 2 is 94.2%, 88.6%, 82.0%, 55.0%, 44.0%, 32.4%, 15.3%, 15.8%, 12.8% and 1.2% at peptide concentrations of 1, 10, 15, 20, 25, 30, 35, 40, 50 and 100 μM. EC50=22.5±0.7 μM. ②The cell viability of Hela cells induced by peptide 2 is 96.1%, 95.4%, 83.8%, 64.2%, 57.7%, 49.5%, 31.5%, 30.2%, 7.8% and 0.2% at peptide concentrations of 1, 10, 15, 20, 25, 30, 35, 40, 50 and 100 μM. EC50=29.8±1 μM. ③The cell viabiity of HDF cells induced by peptide 2 is 96.3%, 89.7%, 89.9%, 92.7%, 95.0%, 93.4%, 93.1%, 96.1%, 88.6% and 67.4% at peptide concentrations of 1, 10, 15, 20, 25, 30, 35, 40, 50 and 100 μM.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • α-helix mainly in 10 mM phosphate buffer, pH7.4

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP21404.

DRAMP21404

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.

• ·Literature 1

• • Title

  • Helminth Defense Molecules as Design Templates for Membrane Active Antibiotics.

• • Pubmed ID

  • 31117348

• • Reference

  • ACS Infect Dis. 2019 May 30. doi: 10.1021/acsinfecdis.9b00157.

• • Author

  • Hammond K, Lewis H, Faruqui N, Russell C, Hoogenboom BW, Ryadnov MG