General Information
-
DRAMP ID
- DRAMP21406
-
Peptide Name
- DGL13K (De Novo Synthesis)
-
Source
- synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- Gkiiklkaslkll
-
Sequence Length
- 13
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.31071184] Gram-positive bacteria:Staphylococcus aureus Xen36(MIC=2.6μg/ml), Staphylococcus aureus USA300(MIC=5.2μg/ml);
- Gram-negative bacteria: Pseudomonas aeruginosa (MIC=5.2μg/ml)
-
Hemolytic Activity
-
- [Ref.31071184] LD50=950μg/ml against human blood cells
-
Cytotoxicity
-
- [Ref.31071184] The cytotoxicity of D-GL13K on HEK cells is 1.5%, 0.9%, 2.1%, 1.5%, 34.2%, 32.4% and 41.1% at peptide concentrations of 1, 3.9, 15.6, 62.5, 250 and 1000 μg/mL, while the cytotoxicity of PMX on HEK cells (Control samples) is 1.2%, 1.2%, 6.6%, 3.6%, 39.6%, 47.1% and 68.5% at peptide concentrations of 1, 3.9, 15.6, 62.5, 250 and 1000 μg/mL.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- Mixed (L-Gly1)
-
Structure
- Unknown
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21406.
Physicochemical Information
-
Formula
- C65H122N16O12
Absent Amino Acids
- CDEFHMNPQRSTVWY
Common Amino Acids
- KL
Mass
- 1424.88
PI
- 10.48
Basic Residues
- 4
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +4
-
Boman Index
- 1007
Hydrophobicity
- 0.769
Aliphatic Index
- 187.69
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 1
DRAMP21406
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.
Literature Information
- ·Literature 1
-
Title
- In vivo activity and low toxicity of the second-generation antimicrobial peptide DGL13K.
-
Pubmed ID
- 31071184
-
Reference
- PLoS One. 2019 May 9;14(5):e0216669. doi: 10.1371/journal.pone.0216669. eCollection 2019.
-
Author
- Gorr SU, Flory CM, Schumacher RJ