• • DRAMP ID

  • DRAMP21416

• • Peptide Name

  • WV (De Novo Synthesis)

• • Source

  • synthetic construct

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • WVKAAAKAAAKVW

• • Sequence Length

  • 13

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.30897850] Gram-positive bacteria:Staphylococcus aureus ATCC 27853(MIC>128μM), Staphylococcus epidermidis ATCC 12228(MIC>128μM), Staphylococcus faecalis ATCC 29212(MIC=64μM), Bacillus subtilis CMCC 63501(MIC>128μM);
    • Gram-negative bacteria:Escherichia coli ATCC 25922 (MIC=32μM), Escherichia coli UB 1005 (MIC>128μM), Pseudomonas aeruginosa ATCC 27853(MIC>128μM), Salmonella typhimurium ATCC 14028(MIC>128μM)

• • Hemolytic Activity

  • • [Ref.30897850] 1.5% hemolysis at 256μM against human red blood cells

• • Cytotoxicity

  • • [Ref.30897850] ①The cell viability of RAW 264.7 induced by WV is 102.5%, 93.5%, 97.8%, 95.7%, 99.3%, 99.6%, 93.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM, while the cytotoxicity of WV on RAW 264.7 cells (Control samples) is 93.1%, 67.8%, 37.7%, 17.8%, 0%, 1.4% and 1.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM.②The cell viability of HEK293T induced by WV is 115.2%, 104.3%, 114.5%, 107.6%, 105.8%, 108.0% and 93.8% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM, while the cytotoxicity of WV on HEK293T cells (Control samples) is 101.4%, 80.1%, 31.9%, 1.4%, 1.4%, 1.8% and 1.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidaton

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • α-helix predicted by I-TASSER, random coil in PBS, α-helix in SDS

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP21416.

DRAMP21416

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.

• ·Literature 1

• • Title

  • Symmetrical Modification of Minimized Dermaseptins to Extend the Spectrum of Antimicrobials with Endotoxin Neutralization Potency

• • Pubmed ID

  • 30897850

• • Reference

  • Int J Mol Sci. 2019 Mar; 20(6): 1417. Published online 2019 Mar 20. doi: 10.3390/ijms20061417

• • Author

  • Changxuan Shao, Weizhong Li, Peng Tan, Anshan Shan, Xiujing Dou, Deying Ma, Chunyu Liu