General Information
-
DRAMP ID
- DRAMP21419
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Peptide Name
- WW (De Novo Synthesis)
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Source
- synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- WWKAAAKAAAKWW
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Sequence Length
- 13
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.30897850] Gram-positive bacteria:Staphylococcus aureus ATCC 27853(MIC=4μM), Staphylococcus epidermidis ATCC 12228(MIC=4μM), Staphylococcus faecalis ATCC 29212(MIC=4μM), Bacillus subtilis CMCC 63501(MIC=4μM);
- Gram-negative bacteria:Escherichia coli ATCC 25922 (MIC=2μM), Escherichia coli UB 1005 (MIC=4μM), Pseudomonas aeruginosa ATCC 27853(MIC=8μM), Salmonella typhimurium ATCC 14028(MIC=8μM)
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Hemolytic Activity
-
- [Ref.30897850] 24% hemolysis at 256μM against human red blood cells
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Cytotoxicity
-
- [Ref.30897850] ①The cell viability of RAW 264.7 induced by WW is 104.3%, 103.6%, 109.4%, 103.6%, 97.5%, 89.9%, 63.4% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM, while the cytotoxicity of WV on RAW 264.7 cells (Control samples) is 93.1%, 67.8%, 37.7%, 17.8%, 0%, 1.4% and 1.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM.②The cell viability of HEK293T induced by WV is 102.9%, 111.6%, 105.1%, 104.3%, 101.8%, 84.8%, 43.5% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM, while the cytotoxicity of WV on HEK293T cells (Control samples) is 101.4%, 80.1%, 31.9%, 1.4%, 1.4%, 1.8% and 1.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM.
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
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C-terminal Modification
- Amidaton
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- α-helix predicted by I-TASSER, random coil in PBS, α-helix in SDS
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21419.
Physicochemical Information
-
Formula
- C80H108N20O14
Absent Amino Acids
- CDEFGHILMNPQRSTVY
Common Amino Acids
- A
Mass
- 1573.86
PI
- 10.3
Basic Residues
- 3
Acidic Residues
- 0
Hydrophobic Residues
- 10
Net Charge
- +3
-
Boman Index
- 353
Hydrophobicity
- -0.346
Aliphatic Index
- 46.15
Half Life
-
- Mammalian:2.8 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 22000
Absorbance 280nm
- 1833.33
Polar Residues
- 0
DRAMP21419
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.
Literature Information
- ·Literature 1
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Title
- Symmetrical Modification of Minimized Dermaseptins to Extend the Spectrum of Antimicrobials with Endotoxin Neutralization Potency
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Pubmed ID
- 30897850
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Reference
- Int J Mol Sci. 2019 Mar; 20(6): 1417. Published online 2019 Mar 20. doi: 10.3390/ijms20061417
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Author
- Changxuan Shao, Weizhong Li, Peng Tan, Anshan Shan, Xiujing Dou, Deying Ma, Chunyu Liu